Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613174257_s681 AAAAATCACCTGTGGAAGCACAAAATTCCATG(+AGGACA)GATCCAGAGTGGAGAGTGTTCTGTTCTATGC NGG 10 8 17 8 24 0 100 5 10
    240613174255_xarq TTAATGCATAGAACAGAACACTCTCCACTCTG(+AGGACA)GATCCATGGAATTTTGTGCTTCCACAGGTGA NGG 10 8 17 8 24 0 100 5 10
    240613174254_2u6c TGTTAGCGAAACACCTTTAAAGATTTATAAGG(+AGGACA)GATCCCAGGTAATTAGTATATATGAAATTTT NGG 10 8 17 8 24 0 100 5 10
    240613174251_eq7h CAATAAAAATTTCATATATACTAATTACCTGG(+AGGACA)GATCCCTTATAAATCTTTAAAGGTGTTTCGC NGG 10 8 17 8 24 0 100 5 10
    240613174250_6hzr GTCTGAGAGCTCTGCACGGGCACAGATGCTGG(+AGGACA)GTACCTCTTATGGGTGTGGCAGTATTACACG NGG 10 8 17 8 24 0 100 5 10
    240613174248_5kuo TGTTTCGTGTAATACTGCCACACCCATAAGAG(+AGGACA)GTACCCAGCATCTGTGCCCGTGCAGAGCTCT NGG 10 8 17 8 24 0 100 5 10
    240613174247_6qcx GTCTGAGAGCTCTGCACGGGCACAGATGCTGG(+AGGACA)GTACCTCTTATGGGTGTGGCAGTATTACACG NGG 10 8 17 8 24 0 100 5 10
    240613174245_agvb TGTTTCGTGTAATACTGCCACACCCATAAGAG(+AGGACA)GTACCCAGCATCTGTGCCCGTGCAGAGCTCT NGG 10 8 17 8 24 0 100 5 10
    240613174243_eatw TTCAAGCAACATATAAAATGAAGAGTTTCCTG(+AGGACA)GTACCTATCATAGTATTTACATTCCCTTATC NGG 10 8 17 8 24 0 100 5 10
    240613174242_h705 TTTGAGATAAGGGAATGTAAATACTATGATAG(+AGGACA)GTACCAGGAAACTCTTCATTTTATATGTTGC NGG 10 8 17 8 24 0 100 5 10