Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613174329_ojll AAGGCAACACTGTGTGATAGGGATGCTCAGAG(+AGGACA)GTACCATATTTAGACATAAAAAGGAAGAGTG NGG 10 8 17 8 24 0 100 5 10
    240613174327_qrl8 GCTGACACTCTTCCTTTTTATGTCTAAATATG(+AGGACA)GTACCTCTGAGCATCCCTATCACACAGTGTT NGG 10 8 17 8 24 0 100 5 10
    240613174325_p57t ATTATTTTTTCTTACGGTTTATTCAATAGAGG(+AGGACA)GTACCTAGAAAAATATTCAGTGTGATGGTCA NGG 10 8 17 8 24 0 100 5 10
    240613174324_mqxf TATGTTGACCATCACACTGAATATTTTTCTAG(+AGGACA)GTACCCTCTATTGAATAAACCGTAAGAAAAA NGG 10 8 17 8 24 0 100 5 10
    240613174322_osay GATCTTGACATTTCCAAGCCACGGTAGCCATG(+AGGACA)GTACCCCATCCACGTGACATTCTGCAGTATG NGG 10 8 17 8 24 0 100 5 10
    240613174320_f775 AGCACCATACTGCAGAATGTCACGTGGATGGG(+AGGACA)GTACCATGGCTACCGTGGCTTGGAAATGTCA NGG 10 8 17 8 24 0 100 5 10
    240613174318_dd7u TGTCCAAAGAAACACAACCGGGACAAAGCCAG(+AGGACA)GATCCGGTCTTGTCCCGGCCTTCTGATATAC NGG 10 8 17 8 24 0 100 5 10
    240613174317_s2bt ATTCTGTATATCAGAAGGCCGGGACAAGACCG(+AGGACA)GATCCTGGCTTTGTCCCGGTTGTGTTTCTTT NGG 10 8 17 8 24 0 100 5 10
    240613174316_s6n5 GCCACTGTTGGAGGAAGGATGAAACCCTTGAG(+AGGACA)GTACCCTCGGAAGCACAGACACAGCGCATCA NGG 10 8 17 8 24 0 100 5 10
    240613174313_1lko GTCTGTGATGCGCTGTGTCTGTGCTTCCGAGG(+AGGACA)GTACCTCAAGGGTTTCATCCTTCCTCCAACA NGG 10 8 17 8 24 0 100 5 10