Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613174432_961n TTTTTGATGTTGTTCTTTTGTCAAGCCCCCAG(+AGGACA)GTACCAAAAAACAGCGTCCCCAGTCGGGTAA NGG 10 8 17 8 24 0 100 5 10
    240613174431_u53e GTTTCTTACCCGACTGGGGACGCTGTTTTTTG(+AGGACA)GTACCTGGGGGCTTGACAAAAGAACAACATC NGG 10 8 17 8 24 0 100 5 10
    240613174429_uw3h CAGTACATCTCTTAGGTGACAGAGGCCAATGG(+AGGACA)GATCCACACCATTGGCCTCTCCTCTGGTGCT NGG 10 8 17 8 24 0 100 5 10
    240613174428_ppn2 ACATTAGCACCAGAGGAGAGGCCAATGGTGTG(+AGGACA)GATCCCATTGGCCTCTGTCACCTAAGAGATG NGG 10 8 17 8 24 0 100 5 10
    240613174426_l7tp GTTTGGCTGTTTGTTTCTGTTTTGTCAGGGTG(+AGGACA)GATCCAAGAGAATGGGGCATTAGTCAGTCAT NGG 10 8 17 8 24 0 100 5 10
    240613174425_fdnn TGCACATGACTGACTAATGCCCCATTCTCTTG(+AGGACA)GATCCACCCTGACAAAACAGAAACAAACAGC NGG 10 8 17 8 24 0 100 5 10
    240613174423_xlo1 CTCAGGTGGGTAACCTGAGTTCAGAGTCCAAG(+AGGACA)GTACCAGTAGCAACGCAGGCAAACTAGTTGT NGG 10 8 17 8 24 0 100 5 10
    240613174422_ih9e GATGTACAACTAGTTTGCCTGCGTTGCTACTG(+AGGACA)GTACCTTGGACTCTGAACTCAGGTTACCCAC NGG 10 8 17 8 24 0 100 5 10
    240613174420_e3di AGACACACAGAGCGGAGGGGAGGGACAACCTG(+AGGACA)GTACCAGTCTGGAAATCTGATTGGCCCAGCA NGG 10 8 17 8 24 0 100 5 10
    240613174419_tp11 CCATCTGCTGGGCCAATCAGATTTCCAGACTG(+AGGACA)GTACCAGGTTGTCCCTCCCCTCCGCTCTGTG NGG 10 8 17 8 24 0 100 5 10