Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613174402_z77s GACCTCCAAAGGTACAGGTCAGAATAGGGCAG(+AGGACA)GATCCCTTGTGAACTACCCTACCATGTGGGT NGG 10 8 17 8 24 0 100 5 10
    240613174400_lp4l TCAGCACCCACATGGTAGGGTAGTTCACAAGG(+AGGACA)GATCCTGCCCTATTCTGACCTGTACCTTTGG NGG 10 8 17 8 24 0 100 5 10
    240613174359_anoo GGCTGATACACTGAGTGCAAGAGTACTGGAGG(+AGGACA)GATCCAGTGAGTCAAACCAGTAAGCAGTGAG NGG 10 8 17 8 24 0 100 5 10
    240613174357_ms5p CACACCTCACTGCTTACTGGTTTGACTCACTG(+AGGACA)GATCCCTCCAGTACTCTTGCACTCAGTGTAT NGG 10 8 17 8 24 0 100 5 10
    240613174356_wem9 CATTATCTTTGACAATTTAAACAAGATCAAAG(+AGGACA)GTACCCAAAGGGTTCTGGGAGCTAAGATCTC NGG 10 8 17 8 24 0 100 5 10
    240613174354_4pxl CTATGGAGATCTTAGCTCCCAGAACCCTTTGG(+AGGACA)GTACCTTTGATCTTGTTTAAATTGTCAAAGA NGG 10 8 17 8 24 0 100 5 10
    240613174353_v4zv CATTATCTTTGACAATTTAAACAAGATCAAAG(+AGGACA)GTACCCAAAGGGTTCTGGGAGCTAAGATCTC NGG 10 8 17 8 24 0 100 5 10
    240613174351_smif CTATGGAGATCTTAGCTCCCAGAACCCTTTGG(+AGGACA)GTACCTTTGATCTTGTTTAAATTGTCAAAGA NGG 10 8 17 8 24 0 100 5 10
    240613174350_upmh GTTTTCTGAAGTCATGGATTAAGTGATCTGCG(+AGGACA)GATCCACCCAGGCACAGTCAGATCAAAGCGT NGG 10 8 17 8 24 0 100 5 10
    240613174348_no99 TTGCAACGCTTTGATCTGACTGTGCCTGGGTG(+AGGACA)GATCCGCAGATCACTTAATCCATGACTTCAG NGG 10 8 17 8 24 0 100 5 10