Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613174502_uaej GTGACTCACTCTCAGTGGTACTGAGGAGGCAG(+AGGACA)GATCCTCATCTTCCTGTACTGCCCACCTGAC NGG 10 8 17 8 24 0 100 5 10
    240613174501_hgyw AACCGGTCAGGTGGGCAGTACAGGAAGATGAG(+AGGACA)GATCCTGCCTCCTCAGTACCACTGAGAGTGA NGG 10 8 17 8 24 0 100 5 10
    240613174500_oh98 AGCCTTCTCCGGCTGCCTCCTGAAGACACAAG(+AGGACA)GATCCTTCGCACCATGGTACTTTGAAAGCTC NGG 10 8 17 8 24 0 100 5 10
    240613174459_2oxq CAGGTGAGCTTTCAAAGTACCATGGTGCGAAG(+AGGACA)GATCCTTGTGTCTTCAGGAGGCAGCCGGAGA NGG 10 8 17 8 24 0 100 5 10
    240613174457_07vi TTACAGACACGAAGTCATTATACACATTAGTG(+AGGACA)GATCCTTGGGACCACAGGGACCTCGTGCAAT NGG 10 8 17 8 24 0 100 5 10
    240613174455_zu4w AGGACATTGCACGAGGTCCCTGTGGTCCCAAG(+AGGACA)GATCCACTAATGTGTATAATGACTTCGTGTC NGG 10 8 17 8 24 0 100 5 10
    240613174453_dzo6 GACAGAACCTTGCAGATAATGAGCTTTTGGAG(+AGGACA)GATCCTCCAAGCTCACCCAGAATCGGAACGG NGG 10 8 17 8 24 0 100 5 10
    240613174452_r1fs TGATTCCGTTCCGATTCTGGGTGAGCTTGGAG(+AGGACA)GATCCTCCAAAAGCTCATTATCTGCAAGGTT NGG 10 8 17 8 24 0 100 5 10
    240613174450_k0fn ACAGTTTAATTCACTGAGGAAAAAAGGATTTG(+AGGACA)GTACCTAAAATGGCCAAATAATGATGTATTT NGG 10 8 17 8 24 0 100 5 10
    240613174449_x24l ACTTTAAATACATCATTATTTGGCCATTTTAG(+AGGACA)GTACCAAATCCTTTTTTCCTCAGTGAATTAA NGG 10 8 17 8 24 0 100 5 10