Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613174550_cgwg CTTAGAAGGGTCACTGGGCACAGCAGGGCCTG(+AGGACA)GATCCATCTGCCACCACAGCATCAAGACAAG NGG 10 8 17 8 24 0 100 5 10
    240613174549_izwa CTCTGCTTGTCTTGATGCTGTGGTGGCAGATG(+AGGACA)GATCCAGGCCCTGCTGTGCCCAGTGACCCTT NGG 10 8 17 8 24 0 100 5 10
    240613174548_i6o4 ACCACAGTCTCACAAACAGAGAGGTAAACACG(+AGGACA)GATCCTTGCTTGCCCTCGGCTGGCTTTCTCC NGG 10 8 17 8 24 0 100 5 10
    240613174546_43zu CGAGAGGAGAAAGCCAGCCGAGGGCAAGCAAG(+AGGACA)GATCCGTGTTTACCTCTCTGTTTGTGAGACT NGG 10 8 17 8 24 0 100 5 10
    240613174545_pmo1 AGGGTGCCCATGGATGCCACAGGAAGGCATGG(+AGGACA)GATCCCTTGGAGCTTGAGTCCCAGGCAATTT NGG 10 8 17 8 24 0 100 5 10
    240613174542_qoqf CACCTAAATTGCCTGGGACTCAAGCTCCAAGG(+AGGACA)GATCCCATGCCTTCCTGTGGCATCCATGGGC NGG 10 8 17 8 24 0 100 5 10
    240613174541_ghk1 CTAGGGTTTTCACCTAGCCTCTGGTAGCTAAG(+AGGACA)GATCCCACCACATCCTTCATATTCCCAGTCA NGG 10 8 17 8 24 0 100 5 10
    240613174539_t7vj TACAGTGACTGGGAATATGAAGGATGTGGTGG(+AGGACA)GATCCTTAGCTACCAGAGGCTAGGTGAAAAC NGG 10 8 17 8 24 0 100 5 10
    240613174537_lwpg GTAGCTCTAGAAACATTTTTAAAAGGTGAGGG(+AGGACA)GATCCCACCTCATCCCATTTTTTAAGTGTAA NGG 10 8 17 8 24 0 100 5 10
    240613174535_pn2o ACCATTTACACTTAAAAAATGGGATGAGGTGG(+AGGACA)GATCCCCTCACCTTTTAAAAATGTTTCTAGA NGG 10 8 17 8 24 0 100 5 10