Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613174622_yhvo GAGGCCAAAATTAGTTTTTGCTACTTGATTTG(+AGGACA)GTACCTTAGGGTTTGGGCTCCTCTGGTTTTT NGG 10 8 17 8 24 0 100 5 10
    240613174619_q5yo CAAGAAAAAACCAGAGGAGCCCAAACCCTAAG(+AGGACA)GTACCAAATCAAGTAGCAAAAACTAATTTTG NGG 10 8 17 8 24 0 100 5 10
    240613174618_wh5g GATAGCAAAACAAAGGCACCTCTACACTCCAG(+AGGACA)GTACCCCACATACATAAACCATTGTTGATCC NGG 10 8 17 8 24 0 100 5 10
    240613174616_m40v CCTACGGATCAACAATGGTTTATGTATGTGGG(+AGGACA)GTACCTGGAGTGTAGAGGTGCCTTTGTTTTG NGG 10 8 17 8 24 0 100 5 10
    240613174615_tau9 ATTGTCATGGGGCTCAGGGATACAGATAACAG(+AGGACA)GATCCTAGAAGCAGGCGTGATATTCCAAGGA NGG 10 8 17 8 24 0 100 5 10
    240613174613_1yj2 CCTGGTCCTTGGAATATCACGCCTGCTTCTAG(+AGGACA)GATCCTGTTATCTGTATCCCTGAGCCCCATG NGG 10 8 17 8 24 0 100 5 10
    240613174612_j7sa GAGGCCAGGCAGGGCCTTGGGCCCAGGAACAG(+AGGACA)GTACCTTGAGAGTGAGAGCTACCCATTGCCT NGG 10 8 17 8 24 0 100 5 10
    240613174611_ai22 GCCACAGGCAATGGGTAGCTCTCACTCTCAAG(+AGGACA)GTACCTGTTCCTGGGCCCAAGGCCCTGCCTG NGG 10 8 17 8 24 0 100 5 10
    240613174609_dh28 GCGTCCTCTGGGACCTTCTTAGATGCTGTAAG(+AGGACA)GTACCTACATACAGACTAAATGTGCAAGCAC NGG 10 8 17 8 24 0 100 5 10
    240613174608_y7v8 TCAAGGTGCTTGCACATTTAGTCTGTATGTAG(+AGGACA)GTACCTTACAGCATCTAAGAAGGTCCCAGAG NGG 10 8 17 8 24 0 100 5 10