Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613174639_jpca AGATTAAAACCCTCTGCAAGCGCACAGCCTTG(+AGGACA)GTACCGCCTCTCATCTCAGTTAACAGTTAGA NGG 10 8 17 8 24 0 100 5 10
    240613174637_a93d TGTCATCTAACTGTTAACTGAGATGAGAGGCG(+AGGACA)GTACCAAGGCTGTGCGCTTGCAGAGGGTTTT NGG 10 8 17 8 24 0 100 5 10
    240613174635_pw46 ACAGTGTGCTCCTGTCTCTGTCACAGTTCCCG(+AGGACA)GATCCCTCAGCAGCCCTGTGTGTTGATAAAG NGG 10 8 17 8 24 0 100 5 10
    240613174633_n9md GCAAACTTTATCAACACACAGGGCTGCTGAGG(+AGGACA)GATCCGGGAACTGTGACAGAGACAGGAGCAC NGG 10 8 17 8 24 0 100 5 10
    240613174632_k7i2 AGTACAAGGGCCCTCAGAAGGTGGAGGCATTG(+AGGACA)GATCCCCTGGAGCTGGAGTTATGGATATGGC NGG 10 8 17 8 24 0 100 5 10
    240613174629_kkdy CGGCAGCCATATCCATAACTCCAGCTCCAGGG(+AGGACA)GATCCAATGCCTCCACCTTCTGAGGGCCCTT NGG 10 8 17 8 24 0 100 5 10
    240613174627_ypdo AGCCACAGCTGGCTTTCCACACAGGTTCTAGG(+AGGACA)GATCCAAACAGGGCCTCAGGTGTATTAGTCA NGG 10 8 17 8 24 0 100 5 10
    240613174626_8fze AACCCTGACTAATACACCTGAGGCCCTGTTTG(+AGGACA)GATCCCTAGAACCTGTGTGGAAAGCCAGCTG NGG 10 8 17 8 24 0 100 5 10
    240613174625_dqxv CATGTTTATAAAATAGCACGTACTGAAAACTG(+AGGACA)GTACCACCCGTGACTGTATAAAGAAGGTAGA NGG 10 8 17 8 24 0 100 5 10
    240613174623_egk8 AGTAATCTACCTTCTTTATACAGTCACGGGTG(+AGGACA)GTACCAGTTTTCAGTACGTGCTATTTTATAA NGG 10 8 17 8 24 0 100 5 10