Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613174750_1lek AGACTGGCAAATAAGTTGGCAGATGCACTTTG(+AGGACA)GTACCCTGTTCCTACTCTAGGCTTTAGAATG NGG 10 8 17 8 24 0 100 5 10
    240613174748_f5vf TGCATCATTCTAAAGCCTAGAGTAGGAACAGG(+AGGACA)GTACCAAAGTGCATCTGCCAACTTATTTGCC NGG 10 8 17 8 24 0 100 5 10
    240613174747_ahsc CCCAGGCACTCAGAAGTGAGGCAGGTAGACTG(+AGGACA)GATCCTTCCTGGGCCAATGAGATGGAGTCGG NGG 10 8 17 8 24 0 100 5 10
    240613174746_h0g1 GCCTGCCGACTCCATCTCATTGGCCCAGGAAG(+AGGACA)GATCCAGTCTACCTGCCTCACTTCTGAGTGC NGG 10 8 17 8 24 0 100 5 10
    240613174744_vq1x GCCTAGGACCCCAGAAGGTGCCATATGATGTG(+AGGACA)GTACCCCGTGCCATGTGATGAGCCGGGGGAG NGG 10 8 17 8 24 0 100 5 10
    240613174742_870c CCACCCTCCCCCGGCTCATCACATGGCACGGG(+AGGACA)GTACCACATCATATGGCACCTTCTGGGGTCC NGG 10 8 17 8 24 0 100 5 10
    240613174740_ot6h ATTCCAGAGACACAGCAGCAGGCAGGGCCCAG(+AGGACA)GTACCTTTGTGTTTTGACCCTCCCAGTTTGC NGG 10 8 17 8 24 0 100 5 10
    240613174739_q6hd ACACAGCAAACTGGGAGGGTCAAAACACAAAG(+AGGACA)GTACCTGGGCCCTGCCTGCTGCTGTGTCTCT NGG 10 8 17 8 24 0 100 5 10
    240613174737_oedf ACACAGCAAACTGGGAAGGACAAAACACAAAG(+AGGACA)GTACCTGGGCCCTGCCTGCTGCTGTGTCTCT NGG 10 8 17 8 24 0 100 5 10
    240613174735_3gzz ATTCCAGAGACACAGCAGCAGGCAGGGCCCAG(+AGGACA)GTACCTTTGTGTTTTGTCCTTCCCAGTTTGC NGG 10 8 17 8 24 0 100 5 10