Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613174840_q12c GCCTTAGGAGTGAAGACAGCCAGGAGAGGCTG(+AGGACA)GTACCGGCCCGCCTCCCTCACGGAGTCTCCT NGG 10 8 17 8 24 0 100 5 10
    240613174838_ampt TCTGCAGGAGACTCCGTGAGGGAGGCGGGCCG(+AGGACA)GTACCAGCCTCTCCTGGCTGTCTTCACTCCT NGG 10 8 17 8 24 0 100 5 10
    240613174836_6ybe AACTCCATTACCTGTGGGTAGTAGCCATAGGG(+AGGACA)GTACCAATAGAAATGGCTCTCTGCTAGGTTG NGG 10 8 17 8 24 0 100 5 10
    240613174835_bw2b TGACACAACCTAGCAGAGAGCCATTTCTATTG(+AGGACA)GTACCCCTATGGCTACTACCCACAGGTAATG NGG 10 8 17 8 24 0 100 5 10
    240613174833_6x7r GTGAACGGGTAATTACAATCTTTTATGGCCTG(+AGGACA)GATCCTTACCTGTTTAAACAGCGTTCACTGA NGG 10 8 17 8 24 0 100 5 10
    240613174832_f91q CCACCTCAGTGAACGCTGTTTAAACAGGTAAG(+AGGACA)GATCCAGGCCATAAAAGATTGTAATTACCCG NGG 10 8 17 8 24 0 100 5 10
    240613174830_65t3 GTGTGTCTGAATGTGCGTGTCTTTCATCCACG(+AGGACA)GATCCAAGGGAACCAGGGCTGTGGCTGGCAG NGG 10 8 17 8 24 0 100 5 10
    240613174827_vlr6 TGGCGCTGCCAGCCACAGCCCTGGTTCCCTTG(+AGGACA)GATCCGTGGATGAAAGACACGCACATTCAGA NGG 10 8 17 8 24 0 100 5 10
    240613174825_za10 CCTTTACTTTGACCTGGCCTGCCTGCTCCCAG(+AGGACA)GATCCTGCCACACGTCTCTCCCTTTCCTGCT NGG 10 8 17 8 24 0 100 5 10
    240613174823_3qpq TCCAGAGCAGGAAAGGGAGAGACGTGTGGCAG(+AGGACA)GATCCTGGGAGCAGGCAGGCCAGGTCAAAGT NGG 10 8 17 8 24 0 100 5 10