Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613174822_fffu AGCAACAGGCAGCAAGCTACATTGCCTGTAAG(+AGGACA)GTACCATGATCTCAGAATCCTGCTCACACAA NGG 10 8 17 8 24 0 100 5 10
    240613174820_e3fp TTTCTTTGTGTGAGCAGGATTCTGAGATCATG(+AGGACA)GTACCTTACAGGCAATGTAGCTTGCTGCCTG NGG 10 8 17 8 24 0 100 5 10
    240613174818_061n TCCTCAGAAAATTGGACATAGTGCTACCGGAG(+AGGACA)GATCCAGCAATACCTCTCCTGGGCATATATC NGG 10 8 17 8 24 0 100 5 10
    240613174817_7bnq TTCTGGATATATGCCCAGGAGAGGTATTGCTG(+AGGACA)GATCCTCCGGTAGCACTATGTCCAATTTTCT NGG 10 8 17 8 24 0 100 5 10
    240613174814_zfdu GGATGGACCATGTAGAGACTGCCATATCCAGG(+AGGACA)GATCCACCCCATAATCAGCATCCAAACGCTG NGG 10 8 17 8 24 0 100 5 10
    240613174813_1jeu GGTGTCAGCGTTTGGATGCTGATTATGGGGTG(+AGGACA)GATCCCTGGATATGGCAGTCTCTACATGGTC NGG 10 8 17 8 24 0 100 5 10
    240613174811_d5yu TAGTTCACTGCCAACCATTGGACTGAGCACGG(+AGGACA)GATCCCGAATGGGGTCCCCAATGGAGGAGTT NGG 10 8 17 8 24 0 100 5 10
    240613174809_ghip TTTCTAACTCCTCCATTGGGGACCCCATTCGG(+AGGACA)GATCCCGTGCTCAGTCCAATGGTTGGCAGTG NGG 10 8 17 8 24 0 100 5 10
    240613174808_u2ux CACAGTCCATATAGTTGTTTCTTTCCAGTCTG(+AGGACA)GATCCTATAATGTATTTTAAGATGTGACAGT NGG 10 8 17 8 24 0 100 5 10
    240613174806_atli ATTGAACTGTCACATCTTAAAATACATTATAG(+AGGACA)GATCCAGACTGGAAAGAAACAACTATATGGA NGG 10 8 17 8 24 0 100 5 10