Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613174913_i4m5 CACATCCACTAGAGTAGTTTGGTTGGGCCAGG(+AGGACA)GTACCCTGGAGCTCGGGAAGGTCTTTCGGAG NGG 10 8 17 8 24 0 100 5 10
    240613174911_s6rq AGATGCTCCGAAAGACCTTCCCGAGCTCCAGG(+AGGACA)GTACCCTGGCCCAACCAAACTACTCTAGTGG NGG 10 8 17 8 24 0 100 5 10
    240613174908_ugc2 TTGAGAGCAGGCCCTGATCATTCTGCCCCAGG(+AGGACA)GATCCTAAGGCCTCACGTAGCACTGCCGGCA NGG 10 8 17 8 24 0 100 5 10
    240613174906_5vud ATCGCTGCCGGCAGTGCTACGTGAGGCCTTAG(+AGGACA)GATCCCTGGGGCAGAATGATCAGGGCCTGCT NGG 10 8 17 8 24 0 100 5 10
    240613174904_z18o AGGAGATATCTCAACTGCTGTACCCAGTAAGG(+AGGACA)GATCCTTCTCTACAGACCCTCACTTGGCTAG NGG 10 8 17 8 24 0 100 5 10
    240613174903_p8e1 TTTCTCTAGCCAAGTGAGGGTCTGTAGAGAAG(+AGGACA)GATCCCTTACTGGGTACAGCAGTTGAGATAT NGG 10 8 17 8 24 0 100 5 10
    240613174901_5tps GGCTCAGTAATTAGAGCACTGGGCCTTCCAGG(+AGGACA)GTACCCAAGTTCAGTTTTCAGTATCTATATG NGG 10 8 17 8 24 0 100 5 10
    240613174859_1yoi GCCATCATATAGATACTGAAAACTGAACTTGG(+AGGACA)GTACCCTGGAAGGCCCAGTGCTCTAATTACT NGG 10 8 17 8 24 0 100 5 10
    240613174858_6j51 CTTTTGATCATTTCTCTAGAGGTCTGGGTGTG(+AGGACA)GATCCTTTTTTGCCTCAGATGTGGGTGGCGA NGG 10 8 17 8 24 0 100 5 10
    240613174856_j8sb GTCTCTCGCCACCCACATCTGAGGCAAAAAAG(+AGGACA)GATCCACACCCAGACCTCTAGAGAAATGATC NGG 10 8 17 8 24 0 100 5 10