Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613174805_p6zy ACTGAGTGTCCTCACCAACTGTACCATCAGTG(+AGGACA)GTACCAAAACTGACTGGTTTTAAAAAACACG NGG 10 8 17 8 24 0 100 5 10
    240613174804_qw62 ATCCACGTGTTTTTTAAAACCAGTCAGTTTTG(+AGGACA)GTACCACTGATGGTACAGTTGGTGAGGACAC NGG 10 8 17 8 24 0 100 5 10
    240613174803_tyzk ATAAGGGCCCTGGACAGGCACAGATACTCTTG(+AGGACA)GATCCCCAGTGGAGATGGATAAGAACCTCAA NGG 10 8 17 8 24 0 100 5 10
    240613174801_zwje CCTCTTTGAGGTTCTTATCCATCTCCACTGGG(+AGGACA)GATCCAAGAGTATCTGTGCCTGTCCAGGGCC NGG 10 8 17 8 24 0 100 5 10
    240613174757_utmg GGATGGACCATCCAGAGACTACCCCACCCGGG(+AGGACA)GATCCATCCCATAATCAGCCACCAAACCCAG NGG 10 8 17 8 24 0 100 5 10
    240613174756_37fz AGTGTCTGGGTTTGGTGGCTGATTATGGGATG(+AGGACA)GATCCCCGGGTGGGGTAGTCTCTGGATGGTC NGG 10 8 17 8 24 0 100 5 10
    240613174755_0kuf CTTGCTGCCAGGAGAGCTTGCTTGGGTATCAG(+AGGACA)GTACCATACCCCTACTGTGCAGAGGCTCTGG NGG 10 8 17 8 24 0 100 5 10
    240613174753_a80c TCTTCCCAGAGCCTCTGCACAGTAGGGGTATG(+AGGACA)GTACCTGATACCCAAGCAAGCTCTCCTGGCA NGG 10 8 17 8 24 0 100 5 10
    240613174752_2ibg ATGACATGATCACCGCCTCACAGTCAGTGATG(+AGGACA)GTACCTTTGTTTTAGTTATGACCAGATAGCT NGG 10 8 17 8 24 0 100 5 10
    240613174751_dpwq ACCCCAGCTATCTGGTCATAACTAAAACAAAG(+AGGACA)GTACCATCACTGACTGTGAGGCGGTGATCAT NGG 10 8 17 8 24 0 100 5 10