Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613174712_66ms ACAGTCTGGGTCTTTATTGATTGACAGTCTGG(+AGGACA)GTACCACCACACACACAGACTTCAGGTATTT NGG 10 8 17 8 24 0 100 5 10
    240613174710_yfs4 TTAAGAAATACCTGAAGTCTGTGTGTGTGGTG(+AGGACA)GTACCCAGACTGTCAATCAATAAAGACCCAG NGG 10 8 17 8 24 0 100 5 10
    240613174709_vjdr GGGAGTCGTCTGCTTCACATGGATATGCGACG(+AGGACA)GTACCTTCTGACAGGGGGTTTTCACCCCTTG NGG 10 8 17 8 24 0 100 5 10
    240613174708_ezuy GTTTTCAAGGGGTGAAAACCCCCTGTCAGAAG(+AGGACA)GTACCGTCGCATATCCATGTGAAGCAGACGA NGG 10 8 17 8 24 0 100 5 10
    240613174706_q0eo GAAGATCTCCCTAGAGGCCACTCCTTTAGGAG(+AGGACA)GTACCAGGAACGCTTTCTGGTGTTGTTAGGG NGG 10 8 17 8 24 0 100 5 10
    240613174704_qium AGCAGCCCTAACAACACCAGAAAGCGTTCCTG(+AGGACA)GTACCTCCTAAAGGAGTGGCCTCTAGGGAGA NGG 10 8 17 8 24 0 100 5 10
    240613174703_3w6c ATGCCAAGCTTAACATACGCGGACCCTCACAG(+AGGACA)GATCCCTAGATCAACCACCCTGAACTGAACT NGG 10 8 17 8 24 0 100 5 10
    240613174701_n0zq GTTCCAGTTCAGTTCAGGGTGGTTGATCTAGG(+AGGACA)GATCCTGTGAGGGTCCGCGTATGTTAAGCTT NGG 10 8 17 8 24 0 100 5 10
    240613174659_non3 GAAGATCTCCCTAGAGGCCACTCCTTTAGGAG(+AGGACA)GTACCAGGAACGCTTTCTGGTGTTGTTAGGG NGG 10 8 17 8 24 0 100 5 10
    240613174657_60kz AGCAGCCCTAACAACACCAGAAAGCGTTCCTG(+AGGACA)GTACCTCCTAAAGGAGTGGCCTCTAGGGAGA NGG 10 8 17 8 24 0 100 5 10