Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613174121_m2m8 GGTGTCAGCGTTTGGATGCTGATTATGGGGTG(+AGGACA)GATCCCTGGATATGGCAGTCTCTACATGGTC NGG 10 8 17 8 24 0 100 5 10
    240613174119_6flo AGTGATGACTCAGATGACGACACTTTATAATG(+AGGACA)GTACCTGGTTGGTGGACAACTCACACAAACT NGG 10 8 17 8 24 0 100 5 10
    240613174117_gbse AACACAGTTTGTGTGAGTTGTCCACCAACCAG(+AGGACA)GTACCATTATAAAGTGTCGTCATCTGAGTCA NGG 10 8 17 8 24 0 100 5 10
    240613174115_wz2x CCAGCTGGCTGGATGTGCCCAGATCCTCCTTG(+AGGACA)GTACCCTGTCCCTTTTCCTACAATGATTTTT NGG 10 8 17 8 24 0 100 5 10
    240613174114_nmz1 CATAGAAAAATCATTGTAGGAAAAGGGACAGG(+AGGACA)GTACCAAGGAGGATCTGGGCACATCCAGCCA NGG 10 8 17 8 24 0 100 5 10
    240613174112_uup9 AATGAGTATCATAGATGGTCAGGCTGCAGATG(+AGGACA)GATCCCAGAACCTGCAGCTACCACGGACACC NGG 10 8 17 8 24 0 100 5 10
    240613174111_x73o CTCCTGGTGTCCGTGGTAGCTGCAGGTTCTGG(+AGGACA)GATCCATCTGCAGCCTGACCATCTATGATAC NGG 10 8 17 8 24 0 100 5 10
    240613174109_ofpr AATGACCCTCACTTAGGAAGCAGTCATGATAG(+AGGACA)GTACCCAAACTTTTAAACCAGGCAGGAGCAC NGG 10 8 17 8 24 0 100 5 10
    240613174108_zh59 TCACTGTGCTCCTGCCTGGTTTAAAAGTTTGG(+AGGACA)GTACCTATCATGACTGCTTCCTAAGTGAGGG NGG 10 8 17 8 24 0 100 5 10
    240613174106_hphd GCAGACATCTCTACCTCTTAACCCAGAGTTCG(+AGGACA)GATCCCTCGGACTTCCAAAATCACACCTCTG NGG 10 8 17 8 24 0 100 5 10