Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613174020_5pgy AGACTGATACATTGGCTTGCAATCAGTGACGG(+AGGACA)GTACCATTTACTCTGCTCAATGTTCCTCTGG NGG 10 8 17 8 24 0 100 5 10
    240613174018_kmlx GTTAGGCCTACTGTGAAGATTTTTCTTTGTTG(+AGGACA)GTACCTTTCGGAGTTGTCTCAGAAAGGGGTT NGG 10 8 17 8 24 0 100 5 10
    240613174017_nj69 ATCAAAACCCCTTTCTGAGACAACTCCGAAAG(+AGGACA)GTACCAACAAAGAAAAATCTTCACAGTAGGC NGG 10 8 17 8 24 0 100 5 10
    240613174015_7snt ACAACAGGATAACCAGAAGGAGTTCAGGTGGG(+AGGACA)GATCCAATTTTGATGATATCACAGAAGCCAG NGG 10 8 17 8 24 0 100 5 10
    240613174014_rc34 GATCTCTGGCTTCTGTGATATCATCAAAATTG(+AGGACA)GATCCCCACCTGAACTCCTTCTGGTTATCCT NGG 10 8 17 8 24 0 100 5 10
    240613174012_womo AGTTGCCATGTGGGAACTGGGAACTGGAGGTG(+AGGACA)GATCCTCTAGAAGAGCAGCTAGTGCTCTTAA NGG 10 8 17 8 24 0 100 5 10
    240613174010_cycr AGTGGTTAAGAGCACTAGCTGCTCTTCTAGAG(+AGGACA)GATCCACCTCCAGTTCCCAGTTCCCACATGG NGG 10 8 17 8 24 0 100 5 10
    240613174008_jblj TCTGTAACAGAATTTTCAGTAGAAAGGCCAAG(+AGGACA)GATCCTTGATTTGTCAATGGTCACCATAGTG NGG 10 8 17 8 24 0 100 5 10
    240613174007_4n61 CTTATCACTATGGTGACCATTGACAAATCAAG(+AGGACA)GATCCTTGGCCTTTCTACTGAAAATTCTGTT NGG 10 8 17 8 24 0 100 5 10
    240613174006_vieb TTACAGATTGCTTAAGGACCTGGGCAGCTGTG(+AGGACA)GATCCACTGGGCTCCTGGGAATAGATGCAGA NGG 10 8 17 8 24 0 100 5 10