Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613174004_hqru GAAGTTCTGCATCTATTCCCAGGAGCCCAGTG(+AGGACA)GATCCACAGCTGCCCAGGTCCTTAAGCAATC NGG 10 8 17 8 24 0 100 5 10
    240613174003_wx7r TAGTGAAGACAACTCTGACCTTGGCATAAAAG(+AGGACA)GATCCAAGTCAGAATCCCAGCTTGGTCAATT NGG 10 8 17 8 24 0 100 5 10
    240613174002_jpu7 GTATTAATTGACCAAGCTGGGATTCTGACTTG(+AGGACA)GATCCTTTTATGCCAAGGTCAGAGTTGTCTT NGG 10 8 17 8 24 0 100 5 10
    240613174000_0i59 TAGAGCTGCCCACATCACTGATCCTGCTTATG(+AGGACA)GATCCTCTCCTCTCTGTGGATGTACCCTGTG NGG 10 8 17 8 24 0 100 5 10
    240613173959_rm0w CAGGACACAGGGTACATCCACAGAGAGGAGAG(+AGGACA)GATCCATAAGCAGGATCAGTGATGTGGGCAG NGG 10 8 17 8 24 0 100 5 10
    240613173958_crvv TAAAAGGAAAAGGGAAGCCACTGCTACTGGAG(+AGGACA)GATCCCCTTCAGTTTTACAAAAAGCTCATCT NGG 10 8 17 8 24 0 100 5 10
    240613173956_1e7y GTTCAAGATGAGCTTTTTGTAAAACTGAAGGG(+AGGACA)GATCCTCCAGTAGCAGTGGCTTCCCTTTTCC NGG 10 8 17 8 24 0 100 5 10
    240613173954_aqvb GTGCATCTTACAACAGCTAAGCATTTATTAAG(+AGGACA)GATCCTCATGAGATACAAGATTAGGGAGACA NGG 10 8 17 8 24 0 100 5 10
    240613173953_hxh4 CCACATGTCTCCCTAATCTTGTATCTCATGAG(+AGGACA)GATCCTTAATAAATGCTTAGCTGTTGTAAGA NGG 10 8 17 8 24 0 100 5 10
    240613173951_r4ot ATGGTGGCACTAATTTCTGAAATTCATCCAGG(+AGGACA)GATCCAAATACTGTTTTTGAGTTCAGTTTTC NGG 10 8 17 8 24 0 100 5 10