Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613173933_6j5m GCTAGGAACTCACGTCACAGGGTTGTTGTGAG(+AGGACA)GATCCAGTGAGCTGATTGGGTAGAGCACCTA NGG 10 8 17 8 24 0 100 5 10
    240613173931_qgc8 CACTAAAACTGGCTCACCTCATCTAGGTTAGG(+AGGACA)GTACCTATCGTTGAGTTTATTAGACAGTTAG NGG 10 8 17 8 24 0 100 5 10
    240613173930_p4it CAACTCTAACTGTCTAATAAACTCAACGATAG(+AGGACA)GTACCCTAACCTAGATGAGGTGAGCCAGTTT NGG 10 8 17 8 24 0 100 5 10
    240613173928_vu02 TGCTAGAGTTATTGTTACAATAATACAGGCCG(+AGGACA)GTACCTACTGTACAGAGTTAAAACTATATGG NGG 10 8 17 8 24 0 100 5 10
    240613173927_lk24 TAAAGCCATATAGTTTTAACTCTGTACAGTAG(+AGGACA)GTACCGGCCTGTATTATTGTAACAATAACTC NGG 10 8 17 8 24 0 100 5 10
    240613173924_di8m CCCCTTAACACCATCTCCAGCCTCATCCTAGG(+AGGACA)GTACCAGCTCAAAACAATACCGGGAGAAGCA NGG 10 8 17 8 24 0 100 5 10
    240613173923_a7e4 CCGCCTGCTTCTCCCGGTATTGTTTTGAGCTG(+AGGACA)GTACCCTAGGATGAGGCTGGAGATGGTGTTA NGG 10 8 17 8 24 0 100 5 10
    240613173922_tv04 AGCAAGCTCGGGAGCTCCCAGAATCTGCTTAG(+AGGACA)GATCCACTCCAGGGCTTGGAGAGGCGATGAA NGG 10 8 17 8 24 0 100 5 10
    240613173921_mazh GTTTGTTCATCGCCTCTCCAAGCCCTGGAGTG(+AGGACA)GATCCTAAGCAGATTCTGGGAGCTCCCGAGC NGG 10 8 17 8 24 0 100 5 10
    240613173919_qwzh AGACCTGGATCTCACTTGGATAAGAGTTCTGG(+AGGACA)GATCCTGCCATGATCCTTGAGAAAGAAAAAT NGG 10 8 17 8 24 0 100 5 10