Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613173831_j9p5 ACAGCCAGAGCAGGGTTGTGTGTTGTTTTGGG(+AGGACA)GTACCACACTAGCCCCTCTTTCGAACTCCAC NGG 10 8 17 8 24 0 100 5 10
    240613173830_brsy CTATTCTCCTTCCAGCCAGCCAGAAATCCTCG(+AGGACA)GTACCTGCACCAGTGACCCAGCAGTCGCACA NGG 10 8 17 8 24 0 100 5 10
    240613173828_4h17 CTCACTGTGCGACTGCTGGGTCACTGGTGCAG(+AGGACA)GTACCGAGGATTTCTGGCTGGCTGGAAGGAG NGG 10 8 17 8 24 0 100 5 10
    240613173827_1o0h AACATTCCAGTTTCCCACAGTCTTATCTTTGG(+AGGACA)GTACCGTTTACAAACTCATGAGGTTGACCTC NGG 10 8 17 8 24 0 100 5 10
    240613173825_34k1 TTACAGAGGTCAACCTCATGAGTTTGTAAACG(+AGGACA)GTACCCAAAGATAAGACTGTGGGAAACTGGA NGG 10 8 17 8 24 0 100 5 10
    240613173823_3upi CAGAAAGATGGAAACGATCAGGGACAGCCCAG(+AGGACA)GATCCCTGATGAGAACTCATGATTTTTTGCG NGG 10 8 17 8 24 0 100 5 10
    240613173821_mwe3 AATGGCGCAAAAAATCATGAGTTCTCATCAGG(+AGGACA)GATCCTGGGCTGTCCCTGATCGTTTCCATCT NGG 10 8 17 8 24 0 100 5 10
    240613173820_dp9i CCCTAGTTTCTGCCCTCCACACATCATCAGTG(+AGGACA)GTACCTTAAGTCTTATTCCTGCCAAAAGGAA NGG 10 8 17 8 24 0 100 5 10
    240613173819_u5d0 GATCTTTCCTTTTGGCAGGAATAAGACTTAAG(+AGGACA)GTACCACTGATGATGTGTGGAGGGCAGAAAC NGG 10 8 17 8 24 0 100 5 10
    240613173818_jay0 CTGTTGCCTGTGAAGGTCAGAAAAGGGCAGTG(+AGGACA)GATCCCATGAGGCTGGATTTATAGATGGTTG NGG 10 8 17 8 24 0 100 5 10