Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613173728_7kzx TGCACCTGTTGGGCAGTTGCATCTTTCGAGAG(+AGGACA)GATCCCTGGTTCTCTCGAACTGTGAGAGCCA NGG 10 8 17 8 24 0 100 5 10
    240613173727_k4o9 CTGCCACCTGGAACGTGGTGGGATTTGGTTTG(+AGGACA)GATCCAAATACTGAGACCCTAGGAAACTTCA NGG 10 8 17 8 24 0 100 5 10
    240613173726_kndi GTCCCTGAAGTTTCCTAGGGTCTCAGTATTTG(+AGGACA)GATCCAAACCAAATCCCACCACGTTCCAGGT NGG 10 8 17 8 24 0 100 5 10
    240613173725_eajc AGCTGATGGCCAAGTTAGATCCTGGTGACTCG(+AGGACA)GATCCAGGGTCCCTCTCCACGGCAGATGCTC NGG 10 8 17 8 24 0 100 5 10
    240613173722_06fd CTCCAGAGCATCTGCCGTGGAGAGGGACCCTG(+AGGACA)GATCCGAGTCACCAGGATCTAACTTGGCCAT NGG 10 8 17 8 24 0 100 5 10
    240613173721_77jc AACTAATTTATTTTTGTCACTTCTAGATGGTG(+AGGACA)GTACCTCTTATAAAATGTTTGGGGGTAGTTT NGG 10 8 17 8 24 0 100 5 10
    240613173719_j14k ACATCAAACTACCCCCAAACATTTTATAAGAG(+AGGACA)GTACCACCATCTAGAAGTGACAAAAATAAAT NGG 10 8 17 8 24 0 100 5 10
    240613173718_7bql AAACCCTGTATCCATAATAACTTCAGTTCTTG(+AGGACA)GATCCAGGGCTTCTGACAAAGTGAATGATGA NGG 10 8 17 8 24 0 100 5 10
    240613173715_bedj TTGCTTCATCATTCACTTTGTCAGAAGCCCTG(+AGGACA)GATCCAAGAACTGAAGTTATTATGGATACAG NGG 10 8 17 8 24 0 100 5 10
    240613173714_fdp9 TATTTTTAAAATTGTATTGCTGCTCAAGAATG(+AGGACA)GTACCCTTTTGTCAAAAAGGTAGATATTCAT NGG 10 8 17 8 24 0 100 5 10