Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613173624_8hur TAACCCATGTAAGGAGAAAGTCCAGGGCACAG(+AGGACA)GTACCCTATTGCAACTAGGTGTAGGAGATAT NGG 10 8 17 8 24 0 100 5 10
    240613173622_nssg ATTGACTACTTCCCAAATCATACATTCCTGAG(+AGGACA)GTACCTAGAAGGACTTCGGAACCCTGGATTT NGG 10 8 17 8 24 0 100 5 10
    240613173621_0agg AAAAGAAATCCAGGGTTCCGAAGTCCTTCTAG(+AGGACA)GTACCTCAGGAATGTATGATTTGGGAAGTAG NGG 10 8 17 8 24 0 100 5 10
    240613173618_0kfd TGCCACCCCTTTGTCAAGCAGACAGACCTTGG(+AGGACA)GATCCACCCGAAGGACAAGATACCAGAAGCT NGG 10 8 17 8 24 0 100 5 10
    240613173617_kl47 CTTGAAGCTTCTGGTATCTTGTCCTTCGGGTG(+AGGACA)GATCCCAAGGTCTGTCTGCTTGACAAAGGGG NGG 10 8 17 8 24 0 100 5 10
    240613173615_u8cq CCCTCCATAAGGAAACAAGGAAAGACATCAGG(+AGGACA)GTACCTACTTCCTATGCACCGTGGACACAGC NGG 10 8 17 8 24 0 100 5 10
    240613173614_2sbx GCTGGGCTGTGTCCACGGTGCATAGGAAGTAG(+AGGACA)GTACCCTGATGTCTTTCCTTGTTTCCTTATG NGG 10 8 17 8 24 0 100 5 10
    240613173613_q77f TGAGAGATTTGATTGGCAGAAGGAATCAAGAG(+AGGACA)GATCCTGCATTTAGGATGCATAGATGGCTAG NGG 10 8 17 8 24 0 100 5 10
    240613173611_obhp CGTCACTAGCCATCTATGCATCCTAAATGCAG(+AGGACA)GATCCTCTTGATTCCTTCTGCCAATCAAATC NGG 10 8 17 8 24 0 100 5 10
    240613173610_js59 GGTTGTCCTATGACTTCCCCACATGTACCATG(+AGGACA)GTACCCATACATGCACACAGTGTACAAACAC NGG 10 8 17 8 24 0 100 5 10