Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613173539_z7uj ATCTGCTCGCCTCCCTCTAGACTGGCCCACTG(+AGGACA)GATCCATACATTTGCTTATCTGCTCCGATCA NGG 10 8 17 8 24 0 100 5 10
    240613173537_jd5j GCCTGTCATGGTATACCCCTGCTAGCCTTGGG(+AGGACA)GATCCATAAGGAGACCCCTAGCATGGCCCTG NGG 10 8 17 8 24 0 100 5 10
    240613173536_6sfo AAGTACAGGGCCATGCTAGGGGTCTCCTTATG(+AGGACA)GATCCCCAAGGCTAGCAGGGGTATACCATGA NGG 10 8 17 8 24 0 100 5 10
    240613173534_jbwc TGGCACTGTTACCACTTTTGAAAGGTTGTTGG(+AGGACA)GTACCAGGGTAATCCCACTAAATGTGGAGTA NGG 10 8 17 8 24 0 100 5 10
    240613173532_tif0 AAGTATACTCCACATTTAGTGGGATTACCCTG(+AGGACA)GTACCCAACAACCTTTCAAAAGTGGTAACAG NGG 10 8 17 8 24 0 100 5 10
    240613173530_98jr TGCTGTGAACTAGAACCGTGGGTAGAGGCCGG(+AGGACA)GTACCCTGGGGTTTGTGGAGAAGGTGTCACT NGG 10 8 17 8 24 0 100 5 10
    240613173527_jfkh GGCACAGTGACACCTTCTCCACAAACCCCAGG(+AGGACA)GTACCCGGCCTCTACCCACGGTTCTAGTTCA NGG 10 8 17 8 24 0 100 5 10
    240613173525_6iyk CAGCCCCCATCAAGTCCCTGTGCAAACTGGAG(+AGGACA)GTACCAGCTGGCTTCAGGATGGGGCACCATA NGG 10 8 17 8 24 0 100 5 10
    240613173524_b2tz GGAGATATGGTGCCCCATCCTGAAGCCAGCTG(+AGGACA)GTACCTCCAGTTTGCACAGGGACTTGATGGG NGG 10 8 17 8 24 0 100 5 10
    240613173522_9hw3 CACTTGTGCTGTTGGCTCATATTTTCACCTTG(+AGGACA)GATCCTCTTACCTGTTGCCCAAGCCTCTGAG NGG 10 8 17 8 24 0 100 5 10