Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613173608_uttq TTTCTGTGTTTGTACACTGTGTGCATGTATGG(+AGGACA)GTACCATGGTACATGTGGGGAAGTCATAGGA NGG 10 8 17 8 24 0 100 5 10
    240613173606_1xmu TCCTGAGATACTCGGTGCCAATAGAAATAGCG(+AGGACA)GATCCGAACACAGCCCCTTTGTGCCAGCGGA NGG 10 8 17 8 24 0 100 5 10
    240613173605_3ixt CAGAATCCGCTGGCACAAAGGGGCTGTGTTCG(+AGGACA)GATCCGCTATTTCTATTGGCACCGAGTATCT NGG 10 8 17 8 24 0 100 5 10
    240613173604_x0x7 AAGTTCCAGGCCAGCCTGCTCTACATGGTGAG(+AGGACA)GATCCTGTGTCTCAGAAACAAGCAAACAAGC NGG 10 8 17 8 24 0 100 5 10
    240613173603_5ets TGTGTGCTTGTTTGCTTGTTTCTGAGACACAG(+AGGACA)GATCCTCACCATGTAGAGCAGGCTGGCCTGG NGG 10 8 17 8 24 0 100 5 10
    240613173601_bs97 TCCTGCCATGCAGTCTACCTGTTTTGAAAATG(+AGGACA)GATCCACTCTTAACAGAGCCAATACCCATGA NGG 10 8 17 8 24 0 100 5 10
    240613173600_gawr ATATTTCATGGGTATTGGCTCTGTTAAGAGTG(+AGGACA)GATCCATTTTCAAAACAGGTAGACTGCATGG NGG 10 8 17 8 24 0 100 5 10
    240613173559_9i5f GCAAAAACAAACAAAAAACAAACCCCTTTTTG(+AGGACA)GTACCTGCACTGCATCCCTCGGGTGGGGCAG NGG 10 8 17 8 24 0 100 5 10
    240613173558_evtv AGGCACTGCCCCACCCGAGGGATGCAGTGCAG(+AGGACA)GTACCAAAAAGGGGTTTGTTTTTTGTTTGTT NGG 10 8 17 8 24 0 100 5 10
    240613173555_o7bh AGCCAGGGCCTGCAGACCTCTACACATCCTGG(+AGGACA)GATCCAGGAGGGCACAGGGAAGGCCAGGTTG NGG 10 8 17 8 24 0 100 5 10