Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613173449_kkfs TCCAGCACTCCCCCAAGGTGGGGGCCCTGTAG(+AGGACA)GTACCAGAGCCTTAGAGGGGTTGCCTTCCTC NGG 10 8 17 8 24 0 100 5 10
    240613173446_tu9x GCCGGTTTCCGGGCTGGCCCCTCCTACCCCTG(+AGGACA)GATCCCAGACTGTCCTGGATTCCACCCTGGG NGG 10 8 17 8 24 0 100 5 10
    240613173445_zbcn ATGAACCCAGGGTGGAATCCAGGACAGTCTGG(+AGGACA)GATCCAGGGGTAGGAGGGGCCAGCCCGGAAA NGG 10 8 17 8 24 0 100 5 10
    240613173443_wt26 GGTCCCTGCAGGAGGACCCTCACCTTCACCTG(+AGGACA)GATCCGCCTTGGCCTCTACCCTTGCCCAACC NGG 10 8 17 8 24 0 100 5 10
    240613173441_u2x4 TGGGAGGTTGGGCAAGGGTAGAGGCCAAGGCG(+AGGACA)GATCCAGGTGAAGGTGAGGGTCCTCCTGCAG NGG 10 8 17 8 24 0 100 5 10
    240613173439_hzuz TAACAGGCCACATTCTGATCTTCCAACCGCTG(+AGGACA)GATCCCTTCCTCCACCTGGCTTCTCAGCTCC NGG 10 8 17 8 24 0 100 5 10
    240613173438_occ4 CATGGGGAGCTGAGAAGCCAGGTGGAGGAAGG(+AGGACA)GATCCAGCGGTTGGAAGATCAGAATGTGGCC NGG 10 8 17 8 24 0 100 5 10
    240613173436_05n8 AAGAGGAGATTTTTTACAAAGTTTCAGTGTGG(+AGGACA)GATCCCTAGGCACTGCCATCACTCAGCAAGA NGG 10 8 17 8 24 0 100 5 10
    240613173434_hym6 TGACTTCTTGCTGAGTGATGGCAGTGCCTAGG(+AGGACA)GATCCCACACTGAAACTTTGTAAAAAATCTC NGG 10 8 17 8 24 0 100 5 10
    240613173432_ky6s TGGCGCCAACATGACCAGAGTCACTAGGAAAG(+AGGACA)GATCCTAGGTAGCACAGACACAAACCATGTC NGG 10 8 17 8 24 0 100 5 10