Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613173413_4baw CTTTTCTTGATTGATGTAGCTATCTTCGGGTG(+AGGACA)GTACCATCCCTAGGCAGGTAGGTCTGGGCTG NGG 10 8 17 8 24 0 100 5 10
    240613173412_vyxg AGTCAGAAAGAACTCCATCACGAGGTAAGGAG(+AGGACA)GTACCGTGTCCAGGAGCTTGCTCCACAGTTC NGG 10 8 17 8 24 0 100 5 10
    240613173410_za3h TTTGGGAACTGTGGAGCAAGCTCCTGGACACG(+AGGACA)GTACCTCCTTACCTCGTGATGGAGTTCTTTC NGG 10 8 17 8 24 0 100 5 10
    240613173409_9kyh AGACAATCTCCGAAGCAAGAATGGTTTATTCG(+AGGACA)GATCCTTGTTCAGTTATGTTTTGAGCTGACT NGG 10 8 17 8 24 0 100 5 10
    240613173408_r6je CCAAAAGTCAGCTCAAAACATAACTGAACAAG(+AGGACA)GATCCGAATAAACCATTCTTGCTTCGGAGAT NGG 10 8 17 8 24 0 100 5 10
    240613173407_pxpl GCCTCCGTATCCCCTGGAAATGTTTTCAGATG(+AGGACA)GATCCCTTGGGTTCTCTGCTACCTAGTAATC NGG 10 8 17 8 24 0 100 5 10
    240613173404_709y CTGGAGATTACTAGGTAGCAGAGAACCCAAGG(+AGGACA)GATCCATCTGAAAACATTTCCAGGGGATACG NGG 10 8 17 8 24 0 100 5 10
    240613173403_og0i GCCATCGGTGCGGTGCGGTGCGTGGATGCCTG(+AGGACA)GATCCCAGCAGCAGCAGACATGGGGCAGGTG NGG 10 8 17 8 24 0 100 5 10
    240613173401_45p8 GTTTGCACCTGCCCCATGTCTGCTGCTGCTGG(+AGGACA)GATCCAGGCATCCACGCACCGCACCGCACCG NGG 10 8 17 8 24 0 100 5 10
    240613173359_j2l7 ACCACATGGTGGCCCACAACCATATGTAATGG(+AGGACA)GATCCAATGCCCTCTTCTGGTGTGTCTGAAG NGG 10 8 17 8 24 0 100 5 10