Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613173344_6dlc ACATTCCAGCCCAGGAAAGGGTGGGAGCTGTG(+AGGACA)GATCCGTGCTGCCCAGGACCCCTTCCTAGGA NGG 10 8 17 8 24 0 100 5 10
    240613173342_dnti AGTGAGAACAAAGGAAAGAGAATCCAAGTGTG(+AGGACA)GATCCCACCCATCCCAACAACCCTTAAATCT NGG 10 8 17 8 24 0 100 5 10
    240613173340_9ax2 ATTTGAGATTTAAGGGTTGTTGGGATGGGTGG(+AGGACA)GATCCACACTTGGATTCTCTTTCCTTTGTTC NGG 10 8 17 8 24 0 100 5 10
    240613173339_ymoe TCTTTGAGAACCTCAACACGTCTGCCTATCAG(+AGGACA)GATCCATGACGGACCCAGCAATTCTCATCTA NGG 10 8 17 8 24 0 100 5 10
    240613173338_wseh TGGTGTAGATGAGAATTGCTGGGTCCGTCATG(+AGGACA)GATCCTGATAGGCAGACGTGTTGAGGTTCTC NGG 10 8 17 8 24 0 100 5 10
    240613173336_yitu TTGAGTGGGAGGAAAGAAACCAGGTGTGAAGG(+AGGACA)GTACCTATTGATAAAATTAAACAATAACTAT NGG 10 8 17 8 24 0 100 5 10
    240613173335_iu80 GATGTATAGTTATTGTTTAATTTTATCAATAG(+AGGACA)GTACCCTTCACACCTGGTTTCTTTCCTCCCA NGG 10 8 17 8 24 0 100 5 10
    240613173334_5x2d CTCTTATTAGAGTTACATTCACATTTGTAGTG(+AGGACA)GATCCTGTCACTTTTTCATTCTAAGTTTAAT NGG 10 8 17 8 24 0 100 5 10
    240613173332_alnk TTCTAATTAAACTTAGAATGAAAAAGTGACAG(+AGGACA)GATCCACTACAAATGTGAATGTAACTCTAAT NGG 10 8 17 8 24 0 100 5 10
    240613173331_kgo7 ACATTATCATCACTGTTGTAGTTTGAAAGAGG(+AGGACA)GTACCACTGTATAACCTATGATGGCTTCAAA NGG 10 8 17 8 24 0 100 5 10