Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613173329_1gaf GCAAGTTTGAAGCCATCATAGGTTATACAGTG(+AGGACA)GTACCCTCTTTCAAACTACAACAGTGATGAT NGG 10 8 17 8 24 0 100 5 10
    240613173328_jr5m ATTGTCTTTAGACACACCAGGAGAGGACATCG(+AGGACA)GATCCCCATTACAGATGGCTGTGAGCCACCA NGG 10 8 17 8 24 0 100 5 10
    240613173326_uzr4 CTACATGGTGGCTCACAGCCATCTGTAATGGG(+AGGACA)GATCCGATGTCCTCTCCTGGTGTGTCTAAAG NGG 10 8 17 8 24 0 100 5 10
    240613173325_ck8f CAATATTATTGGGAAACAGAGACTTTTTTCTG(+AGGACA)GTACCAGACAAAGCTTAAATAGCCCTATATG NGG 10 8 17 8 24 0 100 5 10
    240613173324_upus TGCTTCATATAGGGCTATTTAAGCTTTGTCTG(+AGGACA)GTACCAGAAAAAAGTCTCTGTTTCCCAATAA NGG 10 8 17 8 24 0 100 5 10
    240613173322_2weq TCCAGGGTTGCTGGCTCATGCCTGGACTCTGG(+AGGACA)GATCCTGGCCCTGCAGTCCTCATTGGATATG NGG 10 8 17 8 24 0 100 5 10
    240613173320_uast TCTGGCATATCCAATGAGGACTGCAGGGCCAG(+AGGACA)GATCCCAGAGTCCAGGCATGAGCCAGCAACC NGG 10 8 17 8 24 0 100 5 10
    240613173319_66e3 GGTTCACCATATCAGCTTGATTGGATTAAGAG(+AGGACA)GTACCTGGGGGATCAATAAGGCATATCTCTA NGG 10 8 17 8 24 0 100 5 10
    240613173315_k09k ACACTTAGAGATATGCCTTATTGATCCCCCAG(+AGGACA)GTACCTCTTAATCCAATCAAGCTGATATGGT NGG 10 8 17 8 24 0 100 5 10
    240613173314_8cwk GAATGTGGTTGAACCCCATCTTACAGTTGTGG(+AGGACA)GATCCCAAATGGACTAGTGGTGGGAAGTGAG NGG 10 8 17 8 24 0 100 5 10