Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613173425_hzui AAAACTAAGTAGTATGAGGCCCAAGGAGGAAG(+AGGACA)GTACCCAACCCCGCCTCAGAGGCCCAATGCC NGG 10 8 17 8 24 0 100 5 10
    240613173423_8sj3 TACATGCCTCCCAGTCCCTCCACAGCAACCGG(+AGGACA)GTACCTGGGGATTTTTCCCAAAGGACTCCAT NGG 10 8 17 8 24 0 100 5 10
    240613173419_96d2 TGGCAATGGAGTCCTTTGGGAAAAATCCCCAG(+AGGACA)GTACCCGGTTGCTGTGGAGGGACTGGGAGGC NGG 10 8 17 8 24 0 100 5 10
    240613173418_lggd GGGGCATGCTGGCTTTTCTCTGCCACACTCTG(+AGGACA)GATCCCTGTGCTCTGCTTGCTGGTGAATGAC NGG 10 8 17 8 24 0 100 5 10
    240613173416_ncdc CAGTGGTCATTCACCAGCAAGCAGAGCACAGG(+AGGACA)GATCCAGAGTGTGGCAGAGAAAAGCCAGCAT NGG 10 8 17 8 24 0 100 5 10
    240613173415_n9z4 TTATACAGCCCAGACCTACCTGCCTAGGGATG(+AGGACA)GTACCACCCGAAGATAGCTACATCAATCAAG NGG 10 8 17 8 24 0 100 5 10
    240613173413_4baw CTTTTCTTGATTGATGTAGCTATCTTCGGGTG(+AGGACA)GTACCATCCCTAGGCAGGTAGGTCTGGGCTG NGG 10 8 17 8 24 0 100 5 10
    240613173412_vyxg AGTCAGAAAGAACTCCATCACGAGGTAAGGAG(+AGGACA)GTACCGTGTCCAGGAGCTTGCTCCACAGTTC NGG 10 8 17 8 24 0 100 5 10
    240613173410_za3h TTTGGGAACTGTGGAGCAAGCTCCTGGACACG(+AGGACA)GTACCTCCTTACCTCGTGATGGAGTTCTTTC NGG 10 8 17 8 24 0 100 5 10
    240613173409_9kyh AGACAATCTCCGAAGCAAGAATGGTTTATTCG(+AGGACA)GATCCTTGTTCAGTTATGTTTTGAGCTGACT NGG 10 8 17 8 24 0 100 5 10