Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613173206_ixe7 CCCTGGCTGAGATGCACAACACATGGCAGAAG(+AGGACA)GATCCTTCGGATGCCACCTGTGACGCTTTCC NGG 10 8 17 8 24 0 100 5 10
    240613173205_nmz1 CAAAGTAGGACATTAGCCTTCAGCAAGTTTGG(+AGGACA)GTACCTAAAAGTTTTGTCCTTGTTTTCTCTA NGG 10 8 17 8 24 0 100 5 10
    240613173204_mj4t AAATCTAGAGAAAACAAGGACAAAACTTTTAG(+AGGACA)GTACCCAAACTTGCTGAAGGCTAATGTCCTA NGG 10 8 17 8 24 0 100 5 10
    240613173202_xch0 GATGTGGAGGCAAGACATGGGTAAAGATTCAG(+AGGACA)GATCCCTCCCTCCCTCAGTAAATGGTTTCAA NGG 10 8 17 8 24 0 100 5 10
    240613173200_8eck CAAGGTTGAAACCATTTACTGAGGGAGGGAGG(+AGGACA)GATCCTGAATCTTTACCCATGTCTTGCCTCC NGG 10 8 17 8 24 0 100 5 10
    240613173159_oxzo TTGGTGACTCTGAAGACCAGAAAAGCGTGTTG(+AGGACA)GATCCCAAGAACCAGAGTTGTAGGTGGTTGT NGG 10 8 17 8 24 0 100 5 10
    240613173157_v8tp GGCTCACAACCACCTACAACTCTGGTTCTTGG(+AGGACA)GATCCAACACGCTTTTCTGGTCTTCAGAGTC NGG 10 8 17 8 24 0 100 5 10
    240613173155_wmlt GGGTGGCTTTAGATTTGCTTATTAGCTGAAGG(+AGGACA)GATCCCTCCTGCTTCTGGGATTACAGTCCTC NGG 10 8 17 8 24 0 100 5 10
    240613173153_15x3 CAGCAGAGGACTGTAATCCCAGAAGCAGGAGG(+AGGACA)GATCCCTTCAGCTAATAAGCAAATCTAAAGC NGG 10 8 17 8 24 0 100 5 10
    240613173151_y78i AACCCAGAGGCCAGCCCGCCTGAGTGTGTTGG(+AGGACA)GATCCAATCCCAGCAAGAGCCACCGTTTCCG NGG 10 8 17 8 24 0 100 5 10