Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613173352_rdnl TGCCTTAAAACTTGGCTCATGTGCCATTTGAG(+AGGACA)GATCCTTCCTTTTGCATCTGGAGTCTGCAGT NGG 10 8 17 8 24 0 100 5 10
    240613173351_qqwk AAGGCAACACATTTCCGTTCTGTATGAACATG(+AGGACA)GATCCTTTTCAGGGGAGAGAGCTGCCATGTG NGG 10 8 17 8 24 0 100 5 10
    240613173349_c2jc CTGAGCACATGGCAGCTCTCTCCCCTGAAAAG(+AGGACA)GATCCATGTTCATACAGAACGGAAATGTGTT NGG 10 8 17 8 24 0 100 5 10
    240613173347_x77g TGCTCCTAGTCCCTGATTTTCTGCAGATGAGG(+AGGACA)GTACCTACAGCCTGGGAGGCCACACAGACCT NGG 10 8 17 8 24 0 100 5 10
    240613173346_jr5u ACACAAGGTCTGTGTGGCCTCCCAGGCTGTAG(+AGGACA)GTACCCTCATCTGCAGAAAATCAGGGACTAG NGG 10 8 17 8 24 0 100 5 10
    240613173345_hp7b AGGTTTCCTAGGAAGGGGTCCTGGGCAGCACG(+AGGACA)GATCCACAGCTCCCACCCTTTCCTGGGCTGG NGG 10 8 17 8 24 0 100 5 10
    240613173344_6dlc ACATTCCAGCCCAGGAAAGGGTGGGAGCTGTG(+AGGACA)GATCCGTGCTGCCCAGGACCCCTTCCTAGGA NGG 10 8 17 8 24 0 100 5 10
    240613173342_dnti AGTGAGAACAAAGGAAAGAGAATCCAAGTGTG(+AGGACA)GATCCCACCCATCCCAACAACCCTTAAATCT NGG 10 8 17 8 24 0 100 5 10
    240613173340_9ax2 ATTTGAGATTTAAGGGTTGTTGGGATGGGTGG(+AGGACA)GATCCACACTTGGATTCTCTTTCCTTTGTTC NGG 10 8 17 8 24 0 100 5 10
    240613173339_ymoe TCTTTGAGAACCTCAACACGTCTGCCTATCAG(+AGGACA)GATCCATGACGGACCCAGCAATTCTCATCTA NGG 10 8 17 8 24 0 100 5 10