Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613173048_ggkh ATGATGATGGTGAGACTATCATGAGGATAAAG(+AGGACA)GTACCATGTACCTCACACCCACACTTGCTCT NGG 10 8 17 8 24 0 100 5 10
    240613173047_1zff TGCAAAGAGCAAGTGTGGGTGTGAGGTACATG(+AGGACA)GTACCTTTATCCTCATGATAGTCTCACCATC NGG 10 8 17 8 24 0 100 5 10
    240613173045_mqpp GCATGTGACTGATGAGAATTACAAAAGTCTCG(+AGGACA)GATCCCTAAAAGGCAAGTTGAGAGGTGAGTT NGG 10 8 17 8 24 0 100 5 10
    240613173044_tfp0 GTGAAAACTCACCTCTCAACTTGCCTTTTAGG(+AGGACA)GATCCGAGACTTTTGTAATTCTCATCAGTCA NGG 10 8 17 8 24 0 100 5 10
    240613173042_ili3 AGCACAGGCCCCTCAGAACTCACCTATGTGTG(+AGGACA)GATCCAGCGGCACTTTGCCCAGAAGTGGAAT NGG 10 8 17 8 24 0 100 5 10
    240613173040_34ib TGAGGATTCCACTTCTGGGCAAAGTGCCGCTG(+AGGACA)GATCCACACATAGGTGAGTTCTGAGGGGCCT NGG 10 8 17 8 24 0 100 5 10
    240613173039_jjat ACTTCAAATCCTGTCAGCATCTGGTGGCTATG(+AGGACA)GTACCACCTGTCCCCCATTGCCCAGGTGTCT NGG 10 8 17 8 24 0 100 5 10
    240613173038_9zw2 TGTCCAGACACCTGGGCAATGGGGGACAGGTG(+AGGACA)GTACCATAGCCACCAGATGCTGACAGGATTT NGG 10 8 17 8 24 0 100 5 10
    240613173037_5xbk CCCCCAAAGAATAGCAAATCAGTGTGTTTGTG(+AGGACA)GTACCTACACTACTTTCATTGTAGTCTTCCC NGG 10 8 17 8 24 0 100 5 10
    240613173035_woxi CTTGGGGGAAGACTACAATGAAAGTAGTGTAG(+AGGACA)GTACCACAAACACACTGATTTGCTATTCTTT NGG 10 8 17 8 24 0 100 5 10