Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613172958_scvm CCCGGGGGCCTTAGTTGTGAGGCATGGGTTGG(+AGGACA)GATCCGCCAGCACCAGGGTGTCATCTAGAAC NGG 10 8 17 8 24 0 100 5 10
    240613172956_z8z4 CTTCTGTTCTAGATGACACCCTGGTGCTGGCG(+AGGACA)GATCCCAACCCATGCCTCACAACTAAGGCCC NGG 10 8 17 8 24 0 100 5 10
    240613172954_x7at TAATCCCTTAATTTGGAAGTTTTCTGTTCTGG(+AGGACA)GATCCTCCCAAGAGGTTTTATAAGCTGGTTT NGG 10 8 17 8 24 0 100 5 10
    240613172953_urnn CTCTTAAACCAGCTTATAAAACCTCTTGGGAG(+AGGACA)GATCCCAGAACAGAAAACTTCCAAATTAAGG NGG 10 8 17 8 24 0 100 5 10
    240613172951_qrw1 GTATGTAGTCTGTTTGTCTACACTGTGTATAG(+AGGACA)GATCCACAGTGTAGAAAAGAGTGTTTATAGA NGG 10 8 17 8 24 0 100 5 10
    240613172950_qn05 AATACTCTATAAACACTCTTTTCTACACTGTG(+AGGACA)GATCCTATACACAGTGTAGACAAACAGACTA NGG 10 8 17 8 24 0 100 5 10
    240613172948_eya0 CTGGAAGCAAGCCCCCTCCTGGCATCTCTAGG(+AGGACA)GTACCTGGGCCCACATTCCTGTGGCCCACAA NGG 10 8 17 8 24 0 100 5 10
    240613172946_odbe CCCATTTGTGGGCCACAGGAATGTGGGCCCAG(+AGGACA)GTACCCTAGAGATGCCAGGAGGGGGCTTGCT NGG 10 8 17 8 24 0 100 5 10
    240613172943_8usm TTCCTGGAAGCCTGCCCCCAGCCCAGCCAGGG(+AGGACA)GATCCAGTCAGCAGCGTGGAGGGATTCAAGT NGG 10 8 17 8 24 0 100 5 10
    240613172942_lu8u AAGGCACTTGAATCCCTCCACGCTGCTGACTG(+AGGACA)GATCCCCTGGCTGGGCTGGGGGCAGGCTTCC NGG 10 8 17 8 24 0 100 5 10