Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613172926_tp27 GTGTCCTGGATTTCCTGGATGTTTTGAGTTAG(+AGGACA)GATCCTTTTGCATTTTGTATTTTCTTTGACT NGG 10 8 17 8 24 0 100 5 10
    240613172925_u42e ACAACAGTCAAAGAAAATACAAAATGCAAAAG(+AGGACA)GATCCTAACTCAAAACATCCAGGAAATCCAG NGG 10 8 17 8 24 0 100 5 10
    240613172923_vboj AGGAGTTGTGTTCCACTCACCAGAGGTCTTAG(+AGGACA)GATCCCGTGGGGAATCCTGTGTGGGCCTGTG NGG 10 8 17 8 24 0 100 5 10
    240613172921_s95m ACCCGCACAGGCCCACACAGGATTCCCCACGG(+AGGACA)GATCCTAAGACCTCTGGTGAGTGGAACACAA NGG 10 8 17 8 24 0 100 5 10
    240613172920_py05 GGTGTCAGCGTTTGGATGCTGATTATGGAGTG(+AGGACA)GATCCCTGGATATGGCAGTCTCTACATGGTC NGG 10 8 17 8 24 0 100 5 10
    240613172917_lfb8 GGATGGACCATGTAGAGACTGCCATATCCAGG(+AGGACA)GATCCACTCCATAATCAGCATCCAAACGCTG NGG 10 8 17 8 24 0 100 5 10
    240613172915_mk2u TTCTGGATATATGCCCAAGAGAGGTATTGCTG(+AGGACA)GATCCTCCGGTAGTACTATGTTCAATTTTCT NGG 10 8 17 8 24 0 100 5 10
    240613172913_9nb4 TCCTCAGAAAATTGAACATAGTACTACCGGAG(+AGGACA)GATCCAGCAATACCTCTCTTGGGCATATATC NGG 10 8 17 8 24 0 100 5 10
    240613172911_pmuh AGCCTACTTGGGAGACTGCTGCTCTAAAGCGG(+AGGACA)GTACCCAGACCAGTGAGGGTTTGCCAAAGCG NGG 10 8 17 8 24 0 100 5 10
    240613172910_s89n GATGGCGCTTTGGCAAACCCTCACTGGTCTGG(+AGGACA)GTACCCGCTTTAGAGCAGCAGTCTCCCAAGT NGG 10 8 17 8 24 0 100 5 10