Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613172940_qwi7 GTGCCACCCAGGGTGCTGTTCCCTCTTGTGGG(+AGGACA)GATCCAGAGTAAAACTGGCTCGGCGCCAGGT NGG 10 8 17 8 24 0 100 5 10
    240613172938_37bh ATCCCACCTGGCGCCGAGCCAGTTTTACTCTG(+AGGACA)GATCCCCACAAGAGGGAACAGCACCCTGGGT NGG 10 8 17 8 24 0 100 5 10
    240613172937_u499 TCTTACCCACTCATGGAGTCATGGAGTTACAG(+AGGACA)GTACCTTCCTGAGCAGGGGATGGTAGCTCCT NGG 10 8 17 8 24 0 100 5 10
    240613172936_a3k5 TATTCAGGAGCTACCATCCCCTGCTCAGGAAG(+AGGACA)GTACCTGTAACTCCATGACTCCATGAGTGGG NGG 10 8 17 8 24 0 100 5 10
    240613172933_oluu CAGATCTGCAATTCTCTGTTCTCTCGGCCTGG(+AGGACA)GATCCCAGCTCTCAGCAATCAAAATTGTTGC NGG 10 8 17 8 24 0 100 5 10
    240613172932_wjnd TTGATGCAACAATTTTGATTGCTGAGAGCTGG(+AGGACA)GATCCCAGGCCGAGAGAACAGAGAATTGCAG NGG 10 8 17 8 24 0 100 5 10
    240613172931_vhf0 TGGCACAACTGCCTGCTCCTCTAGTACTAGAG(+AGGACA)GATCCAATGCCCTCAAATTCTCAAGATACTT NGG 10 8 17 8 24 0 100 5 10
    240613172930_m01q TTTTTAAGTATCTTGAGAATTTGAGGGCATTG(+AGGACA)GATCCTCTAGTACTAGAGGAGCAGGCAGTTG NGG 10 8 17 8 24 0 100 5 10
    240613172928_zuj7 CTCCACTCATAATCATGTCTACAGAGCTCTAG(+AGGACA)GTACCTTTTGACAGGTGGAATAAAGTCTCAA NGG 10 8 17 8 24 0 100 5 10
    240613172927_ncsy AAGATTTGAGACTTTATTCCACCTGTCAAAAG(+AGGACA)GTACCTAGAGCTCTGTAGACATGATTATGAG NGG 10 8 17 8 24 0 100 5 10