Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613173016_9vlq CCCTATGCAGACCTTCCTTCCCCTATATCCAG(+AGGACA)GATCCCTGCATCCTTTCCTGGATCTGTTCCA NGG 10 8 17 8 24 0 100 5 10
    240613173014_8s7f AGCCCTGGAACAGATCCAGGAAAGGATGCAGG(+AGGACA)GATCCTGGATATAGGGGAAGGAAGGTCTGCA NGG 10 8 17 8 24 0 100 5 10
    240613173012_wiic GGCCTGGGTTTAAGTGGGGCTTCTTGTCTGGG(+AGGACA)GTACCAAGATAGGGAAAGAAAGAAATAATGA NGG 10 8 17 8 24 0 100 5 10
    240613173010_6cpl GGCAGTCATTATTTCTTTCTTTCCCTATCTTG(+AGGACA)GTACCCCAGACAAGAAGCCCCACTTAAACCC NGG 10 8 17 8 24 0 100 5 10
    240613173009_bovu CACTCAAGAGCTGCCTGGTATGAAGCAAAGAG(+AGGACA)GATCCTGGGCTATTTAAAGCTCTGGTAACCG NGG 10 8 17 8 24 0 100 5 10
    240613173006_ciys CTTCTCGGTTACCAGAGCTTTAAATAGCCCAG(+AGGACA)GATCCTCTTTGCTTCATACCAGGCAGCTCTT NGG 10 8 17 8 24 0 100 5 10
    240613173005_2fcn CTGGGAGCAGGGGCTCAGTGCTGCCGTTCTGG(+AGGACA)GATCCTCCTGTGTCTGATGCCCTGGCCACAG NGG 10 8 17 8 24 0 100 5 10
    240613173003_eowa CCTCTCTGTGGCCAGGGCATCAGACACAGGAG(+AGGACA)GATCCCAGAACGGCAGCACTGAGCCCCTGCT NGG 10 8 17 8 24 0 100 5 10
    240613173001_42f0 GTGCACTTCCATGACTTAAGCATCATTTTTGG(+AGGACA)GATCCCTGCTCACCACCCTTGTTGAGGTTCT NGG 10 8 17 8 24 0 100 5 10
    240613173000_8xuu CACAAAGAACCTCAACAAGGGTGGTGAGCAGG(+AGGACA)GATCCCAAAAATGATGCTTAAGTCATGGAAG NGG 10 8 17 8 24 0 100 5 10