Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613173121_27so GCATGCAGGCCTTCCAGGAGGAACACACGTGG(+AGGACA)GTACCTAGAAACCTAGTGAGGAATGGGAAGT NGG 10 8 17 8 24 0 100 5 10
    240613173120_q0ea TCTAAACTTCCCATTCCTCACTAGGTTTCTAG(+AGGACA)GTACCCACGTGTGTTCCTCCTGGAAGGCCTG NGG 10 8 17 8 24 0 100 5 10
    240613173118_g8jp CCTGTGAAGCAGACTGGGCTTGGTGATGCTTG(+AGGACA)GTACCCACTGAGAGTCAGCTGAGATGCGCTT NGG 10 8 17 8 24 0 100 5 10
    240613173116_6ijn GCTGCAAGCGCATCTCAGCTGACTCTCAGTGG(+AGGACA)GTACCAAGCATCACCAAGCCCAGTCTGCTTC NGG 10 8 17 8 24 0 100 5 10
    240613173113_ti9l AGCCGGAATGGGGTGCTTTGATGTCACCCCAG(+AGGACA)GTACCAGTGAGGGGGCTGGGGCGTTGGTACA NGG 10 8 17 8 24 0 100 5 10
    240613173112_1r9c GGTAGTGTACCAACGCCCCAGCCCCCTCACTG(+AGGACA)GTACCTGGGGTGACATCAAAGCACCCCATTC NGG 10 8 17 8 24 0 100 5 10
    240613173110_8j6a GTCTGAGGTGGGCTAAAAGGAGGGGGACCTGG(+AGGACA)GTACCCCAGTGCTACAGAGATGCTGATGACA NGG 10 8 17 8 24 0 100 5 10
    240613173108_h9oa TTAGCTGTCATCAGCATCTCTGTAGCACTGGG(+AGGACA)GTACCCAGGTCCCCCTCCTTTTAGCCCACCT NGG 10 8 17 8 24 0 100 5 10
    240613173106_pqbo TCTTCTATAATCAAGACTGGCCTAGAACTCCG(+AGGACA)GATCCTCCTGCTCTGTCTCCCAAGTGCTGGA NGG 10 8 17 8 24 0 100 5 10
    240613173104_a43i TGTAATCCAGCACTTGGGAGACAGAGCAGGAG(+AGGACA)GATCCGGAGTTCTAGGCCAGTCTTGATTATA NGG 10 8 17 8 24 0 100 5 10