Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613173034_1n5p ACGTTAGGACTGCCTTGACGCCGTGTACGATG(+AGGACA)GATCCAAGGATAGCTGTGTGCTCCATCAGTG NGG 10 8 17 8 24 0 100 5 10
    240613173032_5w0t AGGACCACTGATGGAGCACACAGCTATCCTTG(+AGGACA)GATCCATCGTACACGGCGTCAAGGCAGTCCT NGG 10 8 17 8 24 0 100 5 10
    240613173030_komw TGACTAAACTATCTATCATTGACACAACTGGG(+AGGACA)GATCCTCCAGGAGCACGGGTCAGTCAAGGAC NGG 10 8 17 8 24 0 100 5 10
    240613173028_2xfg ATTCTGTCCTTGACTGACCCGTGCTCCTGGAG(+AGGACA)GATCCCCAGTTGTGTCAATGATAGATAGTTT NGG 10 8 17 8 24 0 100 5 10
    240613173026_y62x CTCTGCATCTGGCTTTTTACATGGCTGCTGGG(+AGGACA)GATCCAAACTCGGGATATCATGCTTGTACCA NGG 10 8 17 8 24 0 100 5 10
    240613173025_0kze GGCCGTGGTACAAGCATGATATCCCGAGTTTG(+AGGACA)GATCCCCAGCAGCCATGTAAAAAGCCAGATG NGG 10 8 17 8 24 0 100 5 10
    240613173024_h07q TACTGCAAGAAAAAAAAATAAAACATTTCTTG(+AGGACA)GTACCATAACTGTATCATTGTACCCAAATGC NGG 10 8 17 8 24 0 100 5 10
    240613173023_v9q1 CCCTAGCATTTGGGTACAATGATACAGTTATG(+AGGACA)GTACCAAGAAATGTTTTATTTTTTTTTCTTG NGG 10 8 17 8 24 0 100 5 10
    240613173020_crkc TTCCCTGCATGCCTCTTCCCCTGAGCCCAGGG(+AGGACA)GTACCTGGTCCCCATTTCCTACTCCTCTTTT NGG 10 8 17 8 24 0 100 5 10
    240613173018_gnew TTGTTAAAAGAGGAGTAGGAAATGGGGACCAG(+AGGACA)GTACCCCTGGGCTCAGGGGAAGAGGCATGCA NGG 10 8 17 8 24 0 100 5 10