Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613172907_zz7p GTCCTTGGAGCTTTTCTTCTTTCCAGCCCGGG(+AGGACA)GATCCCTTCCTCCTTCAGCTCTTGTCTGCAC NGG 10 8 17 8 24 0 100 5 10
    240613172905_dj54 GTGGGGTGCAGACAAGAGCTGAAGGAGGAAGG(+AGGACA)GATCCCCGGGCTGGAAAGAAGAAAAGCTCCA NGG 10 8 17 8 24 0 100 5 10
    240613172904_vm5p CTCCTTCTACAGTTTCTGAATTTTATAAAGAG(+AGGACA)GTACCCTGTCAGGCAGAAAGTCCAGGACTAA NGG 10 8 17 8 24 0 100 5 10
    240613172902_x0nj GATGCTTAGTCCTGGACTTTCTGCCTGACAGG(+AGGACA)GTACCTCTTTATAAAATTCAGAAACTGTAGA NGG 10 8 17 8 24 0 100 5 10
    240613172901_pahu GGCTAAGTTGAAACAGCCTGTATTCCCTCAAG(+AGGACA)GATCCCTACTCTTGGCCCTCTGACCTAATGA NGG 10 8 17 8 24 0 100 5 10
    240613172859_qm0i ATACCTCATTAGGTCAGAGGGCCAAGAGTAGG(+AGGACA)GATCCTTGAGGGAATACAGGCTGTTTCAACT NGG 10 8 17 8 24 0 100 5 10
    240613172858_b0sh TGTCTCTTAAGTGCTAGGATTTAAGGTGTGTG(+AGGACA)GTACCACATCTGGATGGCATTTGGCTTTTTA NGG 10 8 17 8 24 0 100 5 10
    240613172856_ciaa GAAGTTAAAAAGCCAAATGCCATCCAGATGTG(+AGGACA)GTACCACACACCTTAAATCCTAGCACTTAAG NGG 10 8 17 8 24 0 100 5 10
    240613172855_j62z AAGGGCCAGAGCTGGGGGGCAAGAAGCTTGTG(+AGGACA)GATCCTTCCCCCCATGCACTGTGAAGGACCC NGG 10 8 17 8 24 0 100 5 10
    240613172854_nohd AACGAGGGTCCTTCACAGTGCATGGGGGGAAG(+AGGACA)GATCCACAAGCTTCTTGCCCCCCAGCTCTGG NGG 10 8 17 8 24 0 100 5 10