Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613173108_h9oa TTAGCTGTCATCAGCATCTCTGTAGCACTGGG(+AGGACA)GTACCCAGGTCCCCCTCCTTTTAGCCCACCT NGG 10 8 17 8 24 0 100 5 10
    240613173106_pqbo TCTTCTATAATCAAGACTGGCCTAGAACTCCG(+AGGACA)GATCCTCCTGCTCTGTCTCCCAAGTGCTGGA NGG 10 8 17 8 24 0 100 5 10
    240613173104_a43i TGTAATCCAGCACTTGGGAGACAGAGCAGGAG(+AGGACA)GATCCGGAGTTCTAGGCCAGTCTTGATTATA NGG 10 8 17 8 24 0 100 5 10
    240613173103_j8z8 CTGTATTTTACTGTTTTTTAGGTTTTTCTGAG(+AGGACA)GATCCAGCCCAGTACCTCACACATCCTAAAC NGG 10 8 17 8 24 0 100 5 10
    240613173101_cpw0 TATGTGTTTAGGATGTGTGAGGTACTGGGCTG(+AGGACA)GATCCTCAGAAAAACCTAAAAAACAGTAAAA NGG 10 8 17 8 24 0 100 5 10
    240613173100_4gvh GTAAAAGTTGGTTTCTTTCCTTCTACTGTGTG(+AGGACA)GATCCTGGGATCAAACTAAGGAAGTCAGGCT NGG 10 8 17 8 24 0 100 5 10
    240613173057_8hni TGCCAAGCCTGACTTCCTTAGTTTGATCCCAG(+AGGACA)GATCCACACAGTAGAAGGAAAGAAACCAACT NGG 10 8 17 8 24 0 100 5 10
    240613173056_d1tb TGTCCAGTTAGGTGCCAGCCTGGGCTACATTG(+AGGACA)GATCCTGTCTCTAAAAACCACAAAAAATGAA NGG 10 8 17 8 24 0 100 5 10
    240613173055_hq7f AGGACTTCATTTTTTGTGGTTTTTAGAGACAG(+AGGACA)GATCCAATGTAGCCCAGGCTGGCACCTAACT NGG 10 8 17 8 24 0 100 5 10
    240613173054_b0jt CAGGAACCTAGAACATCTTGTTCACGTGTGTG(+AGGACA)GATCCTGTCTCTAAAAACCACAAAAAATGGA NGG 10 8 17 8 24 0 100 5 10