Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613172801_1jws TCTTCACAATAGCACCCTTCCCAGCAGCCAGG(+AGGACA)GATCCTGGTATCCTGGCTAAACCCATTCCCT NGG 10 8 17 8 24 0 100 5 10
    240613172759_swsc AGGCCAGGGAATGGGTTTAGCCAGGATACCAG(+AGGACA)GATCCCTGGCTGCTGGGAAGGGTGCTATTGT NGG 10 8 17 8 24 0 100 5 10
    240613172757_olgg CCTGAAGGATTCTCCCTAATTCCTTTCCTTCG(+AGGACA)GATCCTCCTCAGGCTCCAGCCACAGTAAATG NGG 10 8 17 8 24 0 100 5 10
    240613172756_ylpm AAATACATTTACTGTGGCTGGAGCCTGAGGAG(+AGGACA)GATCCGAAGGAAAGGAATTAGGGAGAATCCT NGG 10 8 17 8 24 0 100 5 10
    240613172752_lwy2 TCTCTCTGTCCCCACTGTTCACCCAACCAGGG(+AGGACA)GTACCTCTAATGGGAGTGAAGAATCTCTCTC NGG 10 8 17 8 24 0 100 5 10
    240613172751_pexg TAGAGGAGAGAGATTCTTCACTCCCATTAGAG(+AGGACA)GTACCCCTGGTTGGGTGAACAGTGGGGACAG NGG 10 8 17 8 24 0 100 5 10
    240613172750_2hxy CACATCTCCAAAGCCACAGGGACTTGGGTTTG(+AGGACA)GATCCTCCCCATCTGTGTCTACTCCAGCAAT NGG 10 8 17 8 24 0 100 5 10
    240613172748_usip AGAGGATTGCTGGAGTAGACACAGATGGGGAG(+AGGACA)GATCCAAACCCAAGTCCCTGTGGCTTTGGAG NGG 10 8 17 8 24 0 100 5 10
    240613172747_etxg GATGGTAAGGGGACAGAGGGTGGAAGAGTCAG(+AGGACA)GTACCGCCTGTCCCAAGAGACTAGCCAGGGG NGG 10 8 17 8 24 0 100 5 10
    240613172746_vd7i TAGAGCCCCTGGCTAGTCTCTTGGGACAGGCG(+AGGACA)GTACCTGACTCTTCCACCCTCTGTCCCCTTA NGG 10 8 17 8 24 0 100 5 10