Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613172711_rura TGTGACTGAGCTAGCCAGCCAGTAAGTTCAAG(+AGGACA)GATCCTCCTGGCCTCGCGCCCCAACACTGTG NGG 10 8 17 8 24 0 100 5 10
    240613172710_53sv GGGACCACAGTGTTGGGGCGCGAGGCCAGGAG(+AGGACA)GATCCTTGAACTTACTGGCTGGCTAGCTCAG NGG 10 8 17 8 24 0 100 5 10
    240613172708_d69o ATCTTCATTCCTGAGCCACCTTGCCTCCTCTG(+AGGACA)GATCCCTTTTCTAAGAAAACACACTTGGTCT NGG 10 8 17 8 24 0 100 5 10
    240613172706_5da7 AGTGCAGACCAAGTGTGTTTTCTTAGAAAAGG(+AGGACA)GATCCAGAGGAGGCAAGGTGGCTCAGGAATG NGG 10 8 17 8 24 0 100 5 10
    240613172705_u04x AAAATATATCGGCAGTCACTTCTTTTATAAAG(+AGGACA)GTACCATGGGCTACATTACACAATAGTCTCA NGG 10 8 17 8 24 0 100 5 10
    240613172702_xt28 TACATTGAGACTATTGTGTAATGTAGCCCATG(+AGGACA)GTACCTTTATAAAAGAAGTGACTGCCGATAT NGG 10 8 17 8 24 0 100 5 10
    240613172701_wpnx GTTTGATACCCAGGCCTTGCTCTGCTTAGTAG(+AGGACA)GTACCATGGGCCGTTTTAAGAGCTACACATG NGG 10 8 17 8 24 0 100 5 10
    240613172658_gsqy TCTGCCATGTGTAGCTCTTAAAACGGCCCATG(+AGGACA)GTACCTACTAAGCAGAGCAAGGCCTGGGTAT NGG 10 8 17 8 24 0 100 5 10
    240613172657_cile CACTGAGTCAGGGCTCCGGCTTCTGGCTGTGG(+AGGACA)GTACCCACCGCCCAGCTGCAAGAGGGAGTCA NGG 10 8 17 8 24 0 100 5 10
    240613172655_9fkc ATTGGTGACTCCCTCTTGCAGCTGGGCGGTGG(+AGGACA)GTACCCACAGCCAGAAGCCGGAGCCCTGACT NGG 10 8 17 8 24 0 100 5 10