Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613172822_wqze TGCCCCTGGGTCCATGTATCCACCTAGCTCTG(+AGGACA)GTACCCTCCAGAGGTGCCATCTCTACCTCTG NGG 10 8 17 8 24 0 100 5 10
    240613172821_i5si CTCTTCAAAATCCAAAACCTGTGGCCTTACTG(+AGGACA)GTACCAAATTCTCCTTGTAAACATACCTGGC NGG 10 8 17 8 24 0 100 5 10
    240613172819_6fk6 CTGTAGCCAGGTATGTTTACAAGGAGAATTTG(+AGGACA)GTACCAGTAAGGCCACAGGTTTTGGATTTTG NGG 10 8 17 8 24 0 100 5 10
    240613172817_v397 GCTGAGGCAGGCACACTATCCTCACTCCCTCG(+AGGACA)GTACCTGATCTCTAAATCACTTCTCAACTGA NGG 10 8 17 8 24 0 100 5 10
    240613172816_v214 TGGGATCAGTTGAGAAGTGATTTAGAGATCAG(+AGGACA)GTACCGAGGGAGTGAGGATAGTGTGCCTGCC NGG 10 8 17 8 24 0 100 5 10
    240613172813_sf67 CTGGACTACATATAAATTCAGATCAGCCAAGG(+AGGACA)GTACCTAGAAAGACCTATTTCAAAACAAAAA NGG 10 8 17 8 24 0 100 5 10
    240613172812_fad1 CTTGTTTTTTGTTTTGAAATAGGTCTTTCTAG(+AGGACA)GTACCCTTGGCTGATCTGAATTTATATGTAG NGG 10 8 17 8 24 0 100 5 10
    240613172810_b6i6 CTGAGCCTGTGGTCAGCTGACCACAGAGGTGG(+AGGACA)GTACCTGAGTCCCCATGGCCCCCTGGAAGGT NGG 10 8 17 8 24 0 100 5 10
    240613172809_u9xu ATAGGACCTTCCAGGGGGCCATGGGGACTCAG(+AGGACA)GTACCCACCTCTGTGGTCAGCTGACCACAGG NGG 10 8 17 8 24 0 100 5 10
    240613172808_s73s AGGCCTAGGACCAAGGTATCCTCTGTCTGCAG(+AGGACA)GTACCTGACTACCCACAACAGGGAGGCTCGC NGG 10 8 17 8 24 0 100 5 10