Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613172533_i88e TATATTCAGCCCTGCAAAAGAAGTCTTCCCCG(+AGGACA)GATCCCTTAGGCACTTCAGTCACCAGTGTAA NGG 10 8 17 8 24 0 100 5 10
    240613172531_zzo5 ATGGGTTACACTGGTGACTGAAGTGCCTAAGG(+AGGACA)GATCCGGGGAAGACTTCTTTTGCAGGGCTGA NGG 10 8 17 8 24 0 100 5 10
    240613172530_3wnd GCAGAAGAGATGAGTCCATTCCTCAGGGACAG(+AGGACA)GTACCTGTACCTACCCCGATTCCAAACCTAC NGG 10 8 17 8 24 0 100 5 10
    240613172529_2umt GGCAGGTAGGTTTGGAATCGGGGTAGGTACAG(+AGGACA)GTACCTGTCCCTGAGGAATGGACTCATCTCT NGG 10 8 17 8 24 0 100 5 10
    240613172527_x8e0 TGAAAGAGCTCACACTTCCGAGGCGGAAATGG(+AGGACA)GTACCAAAATGAGAGATCAGCAAGATTTCCC NGG 10 8 17 8 24 0 100 5 10
    240613172526_57kc ATCTTGGGAAATCTTGCTGATCTCTCATTTTG(+AGGACA)GTACCCATTTCCGCCTCGGAAGTGTGAGCTC NGG 10 8 17 8 24 0 100 5 10
    240613172524_20wf TTTCTGTAGGAAATGAAATAACTGATTTTGAG(+AGGACA)GTACCAAATAGTGCAATTGGGTAAGACAGGG NGG 10 8 17 8 24 0 100 5 10
    240613172523_s9yz ATAAACCCTGTCTTACCCAATTGCACTATTTG(+AGGACA)GTACCTCAAAATCAGTTATTTCATTTCCTAC NGG 10 8 17 8 24 0 100 5 10
    240613172521_64a0 GCAGGCATCCTGAGAGGTGCCATGACAACCAG(+AGGACA)GATCCTGTTCACAGCCGATCAGTGTGGAGAG NGG 10 8 17 8 24 0 100 5 10
    240613172520_wyo5 GAAGTCTCTCCACACTGATCGGCTGTGAACAG(+AGGACA)GATCCTGGTTGTCATGGCACCTCTCAGGATG NGG 10 8 17 8 24 0 100 5 10