Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613172627_78og GTCAAGGCAGGAAACCGGCGCCGGTAACTAGG(+AGGACA)GATCCAGATAACTGGCAGTGCTGGTGTAGGG NGG 10 8 17 8 24 0 100 5 10
    240613172625_vbze CGACCTGTTCACTGTGAGCTCCATCTTGATCG(+AGGACA)GTACCAGAGGTCAGATTGGGTTTAAGGCTTT NGG 10 8 17 8 24 0 100 5 10
    240613172623_smvt CGAGGAAAGCCTTAAACCCAATCTGACCTCTG(+AGGACA)GTACCGATCAAGATGGAGCTCACAGTGAACA NGG 10 8 17 8 24 0 100 5 10
    240613172622_inh4 CAGGGTCCACCTTCCTCTTCTGTCCTTTGCAG(+AGGACA)GTACCAGACACCCTTATGGTGCACAGTCATA NGG 10 8 17 8 24 0 100 5 10
    240613172621_ribp ATATGTATGACTGTGCACCATAAGGGTGTCTG(+AGGACA)GTACCTGCAAAGGACAGAAGAGGAAGGTGGA NGG 10 8 17 8 24 0 100 5 10
    240613172619_nu7n CCTTTGCAGATGAAGGGCAGAGTCAGTGCTGG(+AGGACA)GATCCCAGCCCTGCCCTCTATTACAGCATAC NGG 10 8 17 8 24 0 100 5 10
    240613172618_0y3g CCTGTGTATGCTGTAATAGAGGGCAGGGCTGG(+AGGACA)GATCCCAGCACTGACTCTGCCCTTCATCTGC NGG 10 8 17 8 24 0 100 5 10
    240613172617_k3wg TATCAGGGCTGGACAATGCCGTAGGAGGAAAG(+AGGACA)GATCCCCAGAGCAGGTGAACGAGTCAGAGAC NGG 10 8 17 8 24 0 100 5 10
    240613172615_x8s0 GGGCTGTCTCTGACTCGTTCACCTGCTCTGGG(+AGGACA)GATCCTTTCCTCCTACGGCATTGTCCAGCCC NGG 10 8 17 8 24 0 100 5 10
    240613172613_3mgy GCTGTCTTCAGACACACCAGAAGAGGGCATCG(+AGGACA)GATCCCACTAGATGGTTGTGAGCCATCATGT NGG 10 8 17 8 24 0 100 5 10