Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613172400_dln3 GATTCCTGATTCCTTCTCAACATGTGTGTAAG(+AGGACA)GATCCTCGACCTGCAAAGTCCATCTCTTTCC NGG 10 8 17 8 24 0 100 5 10
    240613172359_zcmf TTCATGGAAAGAGATGGACTTTGCAGGTCGAG(+AGGACA)GATCCTTACACACATGTTGAGAAGGAATCAG NGG 10 8 17 8 24 0 100 5 10
    240613172358_5dil GCCAACACCAAACGAATCAGTAGCAGCTGCTG(+AGGACA)GATCCAGCAGAAACCACAAGGCTCCACCGAA NGG 10 8 17 8 24 0 100 5 10
    240613172356_yc64 GCAGATTCGGTGGAGCCTTGTGGTTTCTGCTG(+AGGACA)GATCCAGCAGCTGCTACTGATTCGTTTGGTG NGG 10 8 17 8 24 0 100 5 10
    240613172355_6u9v AGATCCTGACTTTGGCTCCAGCTATCAGTTGG(+AGGACA)GATCCTCAACGGACCACAATGAAGGTCCTCC NGG 10 8 17 8 24 0 100 5 10
    240613172353_9yne AAAGTGGAGGACCTTCATTGTGGTCCGTTGAG(+AGGACA)GATCCCAACTGATAGCTGGAGCCAAAGTCAG NGG 10 8 17 8 24 0 100 5 10
    240613172352_i53z TGAAGTGTTGTGGAAGCCAAAAGAGAGTTTTG(+AGGACA)GATCCCCTGGAGTTCCTGGGGAGGAGAGCCA NGG 10 8 17 8 24 0 100 5 10
    240613172350_6dqk GAGTTTGGCTCTCCTCCCCAGGAACTCCAGGG(+AGGACA)GATCCAAAACTCTCTTTTGGCTTCCACAACA NGG 10 8 17 8 24 0 100 5 10
    240613172348_du2v CATGTGCCTTCTGAGTCTAGAAGAGGGTATTG(+AGGACA)GATCCCATGGAGCTGAAATTATAGGCAGTCG NGG 10 8 17 8 24 0 100 5 10
    240613172346_1ddv AGTCACGACTGCCTATAATTTCAGCTCCATGG(+AGGACA)GATCCAATACCCTCTTCTAGACTCAGAAGGC NGG 10 8 17 8 24 0 100 5 10