Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613172415_5alq CACAACTCATTGAAGATAGAGAAGTTGAGCTG(+AGGACA)GTACCTATGTAGAGAGCCTCCACCCCTACAT NGG 10 8 17 8 24 0 100 5 10
    240613172414_j3g0 CTAGAATGTAGGGGTGGAGGCTCTCTACATAG(+AGGACA)GTACCAGCTCAACTTCTCTATCTTCAATGAG NGG 10 8 17 8 24 0 100 5 10
    240613172412_be44 TCTTGTACAAGGAAGTGGAGTGACAGTGGGTG(+AGGACA)GTACCACTTCCCAGTGAAAGGAAGGTTAGGA NGG 10 8 17 8 24 0 100 5 10
    240613172411_lqwa TCTCCTCCTAACCTTCCTTTCACTGGGAAGTG(+AGGACA)GTACCACCCACTGTCACTCCACTTCCTTGTA NGG 10 8 17 8 24 0 100 5 10
    240613172409_59kk ACCTACAGAGCACAGGGCACCTCAGCTATTGG(+AGGACA)GTACCGGAACACAAACACCAGTAACTCCTCA NGG 10 8 17 8 24 0 100 5 10
    240613172408_5ikp CCATGTGAGGAGTTACTGGTGTTTGTGTTCCG(+AGGACA)GTACCCAATAGCTGAGGTGCCCTGTGCTCTG NGG 10 8 17 8 24 0 100 5 10
    240613172406_715d GAAAGAAAACCACATGTCTTGCCTCATCTGTG(+AGGACA)GATCCTGGCCTATAATGTGCACATGTAGACT NGG 10 8 17 8 24 0 100 5 10
    240613172404_nckm CCCTGAGTCTACATGTGCACATTATAGGCCAG(+AGGACA)GATCCACAGATGAGGCAAGACATGTGGTTTT NGG 10 8 17 8 24 0 100 5 10
    240613172403_vhie TGCAGGCACCAGGCACGAATGTGGAGAGCATG(+AGGACA)GATCCGTACAGGCACTCCCATATGCACAAAA NGG 10 8 17 8 24 0 100 5 10
    240613172402_n0f5 TTATTTTTTGTGCATATGGGAGTGCCTGTACG(+AGGACA)GATCCATGCTCTCCACATTCGTGCCTGGTGC NGG 10 8 17 8 24 0 100 5 10