Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613172327_mztt ACCCGCAAGCTCCAGAAACGGCCCCACCTGGG(+AGGACA)GATCCATCCCATTTACAGTTACCAAACCCAG NGG 10 8 17 8 24 0 100 5 10
    240613172325_o2zc AGTGTCTGGGTTTGGTAACTGTAAATGGGATG(+AGGACA)GATCCCCAGGTGGGGCCGTTTCTGGAGCTTG NGG 10 8 17 8 24 0 100 5 10
    240613172324_ls37 AGACCACCTGCTAAAAGGCTCAGAGACAGAGG(+AGGACA)GATCCTGGCTCTTTTATACAACTAAGAAAAA NGG 10 8 17 8 24 0 100 5 10
    240613172322_jthe GTGTCTTTTTCTTAGTTGTATAAAAGAGCCAG(+AGGACA)GATCCCTCTGTCTCTGAGCCTTTTAGCAGGT NGG 10 8 17 8 24 0 100 5 10
    240613172320_ilf6 TTCTACACAGAATATTTTCCCCATATTTATTG(+AGGACA)GATCCAACCTGCTTACGGGATCTGTTAGTGC NGG 10 8 17 8 24 0 100 5 10
    240613172319_bffl TTACAGCACTAACAGATCCCGTAAGCAGGTTG(+AGGACA)GATCCAATAAATATGGGGAAAATATTCTGTG NGG 10 8 17 8 24 0 100 5 10
    240613172317_mnnp CTGTGGAGCATTTTCTTGATTGCTAATTGGAG(+AGGACA)GATCCAACCCACCGTGGGTGTTACTATCCCT NGG 10 8 17 8 24 0 100 5 10
    240613172316_vv6t TGCTTAGGGATAGTAACACCCACGGTGGGTTG(+AGGACA)GATCCTCCAATTAGCAATCAAGAAAATGCTC NGG 10 8 17 8 24 0 100 5 10
    240613172314_4arn AGACAGCATCTGTACATCTTATGTGTAAAAAG(+AGGACA)GATCCAGGGGAAGGTTGTGAGCCACATTGTG NGG 10 8 17 8 24 0 100 5 10
    240613172311_dxcu GCTCTCACAATGTGGCTCACAACCTTCCCCTG(+AGGACA)GATCCTTTTTACACATAAGATGTACAGATGC NGG 10 8 17 8 24 0 100 5 10