Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613172235_cuto CGTGCTCTTAGGTCGTTTTATTTTACAATCTG(+AGGACA)GATCCGAGGTGGCAGATTTAAGACAGGTTCT NGG 10 8 17 8 24 0 100 5 10
    240613172233_t70n TCTACAGAACCTGTCTTAAATCTGCCACCTCG(+AGGACA)GATCCAGATTGTAAAATAAAACGACCTAAGA NGG 10 8 17 8 24 0 100 5 10
    240613172232_mi0v ATGCAACAGCCTGCAAAGGCCATAGGGTGTTG(+AGGACA)GATCCCTGGAACTGGAGTTACAGGAGGTTGA NGG 10 8 17 8 24 0 100 5 10
    240613172229_3bjt TTTCCTCAACCTCCTGTAACTCCAGTTCCAGG(+AGGACA)GATCCAACACCCTATGGCCTTTGCAGGCTGT NGG 10 8 17 8 24 0 100 5 10
    240613172228_ptbv ACAAAAACCCTCTATTAAACACAACAGAAAAG(+AGGACA)GATCCACAGTGCATCCAGACAGTGGAGTTGG NGG 10 8 17 8 24 0 100 5 10
    240613172226_asam GGCTGCCAACTCCACTGTCTGGATGCACTGTG(+AGGACA)GATCCTTTTCTGTTGTGTTTAATAGAGGGTT NGG 10 8 17 8 24 0 100 5 10
    240613172224_eaej AGCTAACACCATCTGCAGCTCCTGTCCCAGGG(+AGGACA)GATCCGCTGTTCTCATGGCCTCTGTGGGCAC NGG 10 8 17 8 24 0 100 5 10
    240613172222_917o GTCCAGTGCCCACAGAGGCCATGAGAACAGCG(+AGGACA)GATCCCCTGGGACAGGAGCTGCAGATGGTGT NGG 10 8 17 8 24 0 100 5 10
    240613172221_lj46 TCAGAAACGAAGTTCACACTGAAGCTCGAAAG(+AGGACA)GTACCAGACAGTGCAGGCTTCCCATCCCATC NGG 10 8 17 8 24 0 100 5 10
    240613172220_kyt8 CGGGGGATGGGATGGGAAGCCTGCACTGTCTG(+AGGACA)GTACCTTTCGAGCTTCAGTGTGAACTTCGTT NGG 10 8 17 8 24 0 100 5 10