Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613172130_fa4j GGGCCTGGGCCAAAACCAGCAAGGTATAGTGG(+AGGACA)GATCCAGGAGGGACTGAGGGAGCCAGGGGCC NGG 10 8 17 8 24 0 100 5 10
    240613172128_t2lc CCTTAGGCCCCTGGCTCCCTCAGTCCCTCCTG(+AGGACA)GATCCCACTATACCTTGCTGGTTTTGGCCCA NGG 10 8 17 8 24 0 100 5 10
    240613172125_1e9p CACGTGTCCTAATGATGAAGTCAGAGCCCCGG(+AGGACA)GATCCCAGCACACACCGTGACACAGCCCAGC NGG 10 8 17 8 24 0 100 5 10
    240613172123_8m94 CAGTGGCTGGGCTGTGTCACGGTGTGTGCTGG(+AGGACA)GATCCCGGGGCTCTGACTTCATCATTAGGAC NGG 10 8 17 8 24 0 100 5 10
    240613172122_lay9 CCGTCAATGCCGCCTCAGGATGCAGGTGTGTG(+AGGACA)GTACCATGGCCTTGGGCAGCCCGTTGGCCTG NGG 10 8 17 8 24 0 100 5 10
    240613172120_jbxw CATCTCAGGCCAACGGGCTGCCCAAGGCCATG(+AGGACA)GTACCACACACCTGCATCCTGAGGCGGCATT NGG 10 8 17 8 24 0 100 5 10
    240613172119_r9gn AAGCAAATGTAAGGGTATAGGAGCAGCAGCAG(+AGGACA)GATCCTGAGTCACAAGGGCACAATGCCAGCT NGG 10 8 17 8 24 0 100 5 10
    240613172118_5g26 TCTCCAGCTGGCATTGTGCCCTTGTGACTCAG(+AGGACA)GATCCTGCTGCTGCTCCTATACCCTTACATT NGG 10 8 17 8 24 0 100 5 10
    240613172115_g63j GGCTTCAGTTCTCCCAAAGAGCCAGCCCCTTG(+AGGACA)GATCCAGTGCGCCCAGCCTTTCCCATGTCTA NGG 10 8 17 8 24 0 100 5 10
    240613172114_pgjp CGCAGTAGACATGGGAAAGGCTGGGCGCACTG(+AGGACA)GATCCAAGGGGCTGGCTCTTTGGGAGAACTG NGG 10 8 17 8 24 0 100 5 10