Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613172038_cnxd GAGTGGCATTTGTCTAAGATTCACCAGAACAG(+AGGACA)GATCCTGTCCTCCTAGGGGACCTTCCAAACA NGG 10 8 17 8 24 0 100 5 10
    240613172037_d5qe TCCTATGTTTGGAAGGTCCCCTAGGAGGACAG(+AGGACA)GATCCTGTTCTGGTGAATCTTAGACAAATGC NGG 10 8 17 8 24 0 100 5 10
    240613172035_6r00 CCAGCATCCAAGGTCAACGCAATGGTGGTCTG(+AGGACA)GTACCCAATGGAGACAAATCCCTTTACTTCT NGG 10 8 17 8 24 0 100 5 10
    240613172034_o144 CAAAGAGAAGTAAAGGGATTTGTCTCCATTGG(+AGGACA)GTACCAGACCACCATTGCGTTGACCTTGGAT NGG 10 8 17 8 24 0 100 5 10
    240613172032_0ija CATGTCTCTTAAGTTATTGTGACCTACACTGG(+AGGACA)GTACCGGAGGGGATGGGGGATGGCTTCAGCT NGG 10 8 17 8 24 0 100 5 10
    240613172030_jo4c ACAGCAGCTGAAGCCATCCCCCATCCCCTCCG(+AGGACA)GTACCCAGTGTAGGTCACAATAACTTAAGAG NGG 10 8 17 8 24 0 100 5 10
    240613172029_9356 TGTGGCACTCAAGCATACTGATCCCCGCTCAG(+AGGACA)GTACCACCATTCCCTATGTAGAGGAGGGAAG NGG 10 8 17 8 24 0 100 5 10
    240613172027_5qrt GGGTGCTTCCCTCCTCTACATAGGGAATGGTG(+AGGACA)GTACCTGAGCGGGGATCAGTATGCTTGAGTG NGG 10 8 17 8 24 0 100 5 10
    240613172026_2atu ATAGAACACTCAAAAGGGACGTGAATGTGCAG(+AGGACA)GTACCATGGGTCTGGGGTGACCTGCTTCACA NGG 10 8 17 8 24 0 100 5 10
    240613172023_qrfn ACTTCTGTGAAGCAGGTCACCCCAGACCCATG(+AGGACA)GTACCTGCACATTCACGTCCCTTTTGAGTGT NGG 10 8 17 8 24 0 100 5 10