Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613171948_vbwu AATAAATAAATCTTTAGGAAAAAATCTGCAAG(+AGGACA)GTACCAGATGGAGGATTCGGGGTCAAGGTGA NGG 10 8 17 8 24 0 100 5 10
    240613171947_zred CCACATCACCTTGACCCCGAATCCTCCATCTG(+AGGACA)GTACCTTGCAGATTTTTTCCTAAAGATTTAT NGG 10 8 17 8 24 0 100 5 10
    240613171946_ly8o GCTATGCAGAGTCCCATGGTCTCTGCATGTTG(+AGGACA)GTACCTCACCAAGTCCCACTGTGCTCTTGTG NGG 10 8 17 8 24 0 100 5 10
    240613171945_v832 TCTACCACAAGAGCACAGTGGGACTTGGTGAG(+AGGACA)GTACCAACATGCAGAGACCATGGGACTCTGC NGG 10 8 17 8 24 0 100 5 10
    240613171943_r8we CCACCATTGCTTCTGTAACTGCTGGGCGCTGG(+AGGACA)GATCCTGCCCCCAGGAAGTTAAGAGTGGGCA NGG 10 8 17 8 24 0 100 5 10
    240613171941_43xk GCTCCTGCCCACTCTTAACTTCCTGGGGGCAG(+AGGACA)GATCCCAGCGCCCAGCAGTTACAGAAGCAAT NGG 10 8 17 8 24 0 100 5 10
    240613171940_i7no CCTTTTTTAAAGATTTATTATTTTTGAGATAG(+AGGACA)GTACCTCTGGATGGCCTGGAACTTGCCATGT NGG 10 8 17 8 24 0 100 5 10
    240613171937_zgzq GGTCTACATGGCAAGTTCCAGGCCATCCAGAG(+AGGACA)GTACCTATCTCAAAAATAATAAATCTTTAAA NGG 10 8 17 8 24 0 100 5 10
    240613171936_hzxy AGCTTTGAAACTGGGCAAGATCCAGTTCATAG(+AGGACA)GATCCCTTCTCGGGACCATGTCTCAGTTCCT NGG 10 8 17 8 24 0 100 5 10
    240613171935_ri00 GGTGCAGGAACTGAGACATGGTCCCGAGAAGG(+AGGACA)GATCCTATGAACTGGATCTTGCCCAGTTTCA NGG 10 8 17 8 24 0 100 5 10